Home / 4-1BB/TNFRSF9/CD137 / Human 4-1BB/TNFRSF9/CD137 Antibody

Human 4-1BB/TNFRSF9/CD137 Antibody

Catalog #: AF838
6 Citations 1 Review Datasheet / COA / SDS
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AF838
AF838-SP
Detection of Human 4‑1BB/TNFRSF9/ CD137 by Western Blot.
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Citations (6)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human 4-1BB/TNFRSF9/CD137 Antibody Summary

Species Reactivity
Human
Specificity
Detects 4‑1BB/TNFRSF9/CD137 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human 4‑1BB/TNFRSF9/CD137
Leu24-Gln186
Accession # Q07011
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
HEK293T human embryonic kidney cell line transfected with human 4-1BB/TNFRSF9/CD137-eGFP Fusion
Simple Western
20 µg/mL
HDLM‑2 human Hodgkin's lymphoma cells
Flow Cytometry
0.25 µg/106 cells
See below
Immunohistochemistry
5-25 µg/mL
See below
Agonist Activity
Measured by its ability to co-stimulate IFN-gamma secretion by human T cells in the presence of anti-CD3.
The ED50 for this effect is typically ≤ 20 μg/mL.
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
5-15 µg/mL
Immersion fixed human peripheral blood mononuclear cells

Human 4-1BB/TNFRSF9/CD137 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
0.2-0.8 µg/mL 

Use in combination with:

Detection Reagent: Human 4‑1BB/TNFRSF9/CD137 Biotinylated Antibody (Catalog # BAF838)

Standard: Recombinant Human 4-1BB/TNFRSF9 Fc Chimera Protein, CF (Catalog # 838-4B)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human 4-1BB/TNFRSF9/ CD137 antibody by Western Blot. View Larger

Detection of Human 4‑1BB/TNFRSF9/ CD137 by Western Blot. Western blot shows lysates of HEK293T human embryonic kidney cell line either mock transfected or transfected with human 4-1BB/TNFRSF9/ CD137-eGFP Fusion. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human 4-1BB/ TNFRSF9/CD137 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF838) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody ([catalogNumber:HAF017]]). A specific band was detected for 4-1BB/ TNFRSF9/CD137-eGFP Fusion at approximately 50-65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry 4-1BB/TNFRSF9/CD137 antibody in Human Tonsil by Immunohistochemistry (IHC-P). View Larger

4‑1BB/TNFRSF9/CD137 in Human Tonsil. 4-1BB/TNFRSF9/CD137 was detected in immersion fixed paraffin-embedded sections of human tonsil using Goat Anti-Human 4-1BB/TNFRSF9/CD137 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF838) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Flow Cytometry Detection of 4-1BB/TNFRSF9/CD137 antibody in PHA-treated Human T Cells antibody by Flow Cytometry. View Larger

Detection of 4‑1BB/TNFRSF9/CD137 in PHA-treated Human T Cells by Flow Cytometry. Human T cells were treated for 48 hours with 5 µg/mL PHA then stained with Goat Anti-Human 4-1BB/TNFRSF9/ CD137 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF838) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (F0108) and PE-conjugated anti-human CD3 (Catalog # FAB100P). Quad marker was set based on control antibody (AB-108-C) staining.

Simple Western View Larger

Detection of Human 4‑1BB/TNFRSF9/CD137 by Simple WesternTM. Simple Western lane view shows lysates of HDLM‑2 human Hodgkin’s lymphoma cells, loaded at 0.2 mg/mL. A specific band was detected for 4‑1BB/TNFRSF9/CD137 at approximately 58 kDa (as indicated) using 20 µg/mL of Goat Anti-Human 4‑1BB/TNFRSF9/CD137 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF838). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Immunocytochemistry View Larger

Detection of 4‑1BB/TNFRSF9/CD137 in Human Untreated PBMC and PHA Treated Human PBMC. 4‑1BB/TNFRSF9/CD137 was detected in immersion fixed human untreated PBMC cells and PHA treated human PBMC cells using Goat Anti-Human 4‑1BB/TNFRSF9/CD137 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF838) at 15 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm of PHA stimulated cells. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: 4-1BB/TNFRSF9/CD137

4-1BB is an inducible T cell surface protein belonging to the TNF receptor superfamily. It is alternatively known as TNFRSF9, CD137, and ILA. The 255 amino acid human 4-1BB is a type I transmembrane protein having in its extracellular domain four of the cysteine-rich motifs that are characteristic of the TNF receptor superfamily. The 30 kDa glycoprotein exists both as a monomer and as a dimer on T cells. The human and mouse proteins share 60% amino acid identity. 4-1BB is absent from naive T cells, but it is upregulated and continually expressed following T cell activation. The natural ligand, 4-1BBL, is a member of the TNF superfamily and is expressed on activated antigen presenting cells including dendritic cells, macrophages, and B cells. Cross-linking of 4-1BB by 4-1BBL or by agonistic antibodies transmits a potent co-stimulatory signal that enhances the effect of other activating signals such as PHA or anti-CD3 antibodies. 4-1BB signals through the
TFAF2‑NIK pathway resulting in activation of NF-kappa B and ultimately promoting proliferation and survival of T cells.

References
  1. Vinay, D. and B. Kwon (1998) Semin. Immunol. 10:481.
  2. Sica, G. and L. Chen (2000) Adv. Exp. Med. Biol. 465:355.
Entrez Gene IDs
3604 (Human); 21942 (Mouse); 500590 (Rat); 102127961 (Cynomolgus Monkey)
Alternate Names
4-1BB ligand receptor; 41BB; 4-1BB; CD137 antigen; CD137; CD137MGC2172; CDw137; FLJ43501; ILA; ILAhomolog of mouse 4-1BB; induced by lymphocyte activation (ILA); interleukin-activated receptor, homolog of mouse Ly63; receptor protein 4-1BB; T cell antigen ILA; T-cell antigen 4-1BB homolog; T-cell antigen ILA; TNFRSF9; tumor necrosis factor receptor superfamily member 9; tumor necrosis factor receptor superfamily, member 9

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Citations for Human 4-1BB/TNFRSF9/CD137 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Co-stimulation through the CD137/4-1BB pathway protects human melanoma tumor-infiltrating lymphocytes from activation-induced cell death and enhances anti-tumor effector function
    Authors: Jessica Ann Hernandez-Chacon, Yufeng Li, Richard C. Wu, Chantale Bernatchez, Yijun Wang, Jeffrey S. Weber et al.
    Journal of Immunotherapy
  2. Anti-CD137 monoclonal antibody enhances trastuzumab-induced, natural killer cell-mediated cytotoxicity against pancreatic cancer cell lines with low human epidermal growth factor-like receptor 2 expression
    Authors: T Masu, M Atsukawa, K Nakatsuka, M Shimizu, D Miura, T Arai, H Harimoto, C Kondo, K Kaneko, S Futagami, C Kawamoto, H Takahashi, K Iwakiri
    PLoS ONE, 2018-12-31;13(12):e0200664.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Targeting peripheral blood pro-inflammatory cytotoxic lymphocytes by inhibiting CD137 expression: novel potential treatment for COPD.
    Authors: Hodge G, Holmes M, Jersmann H, Reynolds P, Hodge S
    BMC Pulm Med, 2014-05-15;14(0):85.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  4. 4-1BB is superior to CD28 costimulation for generating CD8+ cytotoxic lymphocytes for adoptive immunotherapy.
    Authors: Zhang H, Snyder KM, Suhoski MM, Maus MV, Kapoor V, June CH, Mackall CL
    J. Immunol., 2007-10-01;179(7):4910-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  5. Functional expression of 4-1BB (CD137) in the inflammatory tissue in Crohn's disease.
    Authors: Maerten P, Geboes K, De Hertogh G, Shen C, Cadot P, Bullens DM, Van Assche G, Penninckx F, Rutgeerts P, Ceuppens JL
    Clin. Immunol., 2004-09-01;112(3):239-46.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: Functional Assay, IHC-Fr
  6. 4-1BB Agonism Averts TIL Exhaustion and Licenses PD-1 Blockade in Glioblastoma and Other Intracranial Cancers
    Authors: Woroniecka KI, Rhodin KE, Dechant CA et al.
    Clin. Cancer Res.

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Human 4-1BB/TNFRSF9/CD137 Antibody
By Anonymous on 10/13/2017
Application: Flow Sample Tested: Adult kidney Species: Human