Human 5-HT6 Antibody

Catalog #: MAB9496 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB9496-100
MAB9496-SP

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Human 5-HT6 Antibody Summary

Species Reactivity

Human

Specificity

Detects human 5-HT6 in direct ELISAs.

Source

Recombinant Monoclonal Rabbit IgG Clone # 2157A

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human 5-HT6 peptide
Accession # P50406

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

1-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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5-HT6 in Rat Brain. 5-HT6 was detected in perfusion fixed frozen sections of rat brain (medulla) using Rabbit Anti-Human 5-HT6 Monoclonal Antibody (Catalog # MAB9496) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm of neuronal cell bodies. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: 5-HT6

5-HT6 is a seven transmembrane G-protein coupled 1 receptor for serotonin, with 93% homology between human and rat, and with highest expression in neurons of the striatum, cortex and hippocampus. Expression in cultured primary astrocytes from rats and glia has also been demonstrated for most brain regions. Expression in lymph node, spleen and testis has also been observed. High affinity for psychotropic drugs including tricyclic antidepressants and antipsychotics and high expression in the limbic regions suggest a role for 5-HT6 in emotional behaviors and mental illness. Lower expression of 5-HT6 in cortical layers I-V have been observe in Alzheimer's Disease brains.

Long Name

5-Hydroxytryptamine Receptor 6

Entrez Gene IDs

3362 (Human); 15565 (Mouse); 64354 (Rat)

Alternate Names

5-HT6; 5-hydroxytryptamine (serotonin) receptor 6,5-HT65-HT6R; HTR6; Serotonin receptor 6,5-HT-6,5-hydroxytryptamine receptor 6