Human ADAMTS4 Antibody

Catalog #: MAB43071 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB43071-100
MAB43071-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ADAMTS4 Antibody Summary

Species Reactivity

Human

Specificity

Detects human ADAMTS4 in direct ELISA.

Source

Monoclonal Mouse IgG₁ Clone # 416608

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human ADAMTS4
Phe213-Cys685
Accession # O75173

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Human ADAMTS4 Monoclonal Antibody (Catalog # MAB4307).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human ADAMTS4 DuoSet ELISA Kit (Catalog # DY4307-05) for convenient development of a sandwich ELISA.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

ELISA

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Human ADAMTS4 ELISA Standard Curve. Recombinant Human ADAMTS4 protein was serially diluted 2-fold and captured by Mouse Anti-Human ADAMTS4 Monoclonal Antibody (Catalog # MAB43071) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human ADAMTS4 Monoclonal Antibody (Catalog # MAB4307) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ADAMTS4

ADAMTS4 (a disintegrin and metalloproteinase with thrombospondin motifs 4), also known as aggrecanase-1, is a member of the family of secreted zinc proteases with a multi-domain structure (1‑3). The protein precursors consist of a signal peptide and the following domains: pro, catalytic, disintegrin-like, TS type 1 motif, cysteine-rich, and spacer. It is the only ADAMTS identified that has one TS type I motif. It is an active protease effectively cleaving alpha -2-macroglobulin and aggrecan at multiple sites, and is inhibited by TIMP-3 with inhibition constants in subnanomolar range (4‑6). It receives great attention due to the elevation in its mRNA level after treatment with Interleukin-1 (7). However, in a mouse model of osteoarthritis, ADAMTS4 knock-out mice did not exhibit any significant protective effect (8). ADAMTS4 consists of a signal peptide (aa 1 to 51), a pro domain (aa 52 to 212) and a mature chain (aa 213 to 837) containing the following domains: catalytic (aa 218 to 425), disintegrin (aa 437 to 519), TSP type-1 (aa 520 to 575), Cys-rich (aa 577 to 685) and spacer (aa 686 to 837). The amino acid sequence of rhADAMTS4 is 100%, 97% and 94% identical to that of chimpanzee, dog, and mouse/rat/bovine.

References

Totorella, M. D. et al. (1999) Science 284:1664.
Porter, S. et al. (2005) Biochem. J. 386:15.
Nagase, H. and M. Kashiwagi (2003) Arthritis Res. Ther. 5:94.
Tortorella, M. D. et al. (2004) J. Biol. Chem. 279:17554.
Struglics, A. et al. (2006) Osteoarthritis Cartilage. 14:101.
Kashiwagi, M. et al. (2001) J. Biol. Chem. 276:12501.
Pratta, M. A. et al. (2003) Arthritis Reum. 48:119.
Glasson, S. S. et al. (2004) Arthritis Reum. 50:2547.

Long Name

A Disintegrin-like and Metalloproteinase Domain with Thrombospondin Motifs 4

Entrez Gene IDs

9507 (Human); 66015 (Rat)

Alternate Names

a disintegrin-like and metalloprotease (reprolysin type) with thrombospondintype 1 motif, 4; ADAM metallopeptidase with thrombospondin type 1 motif, 4; ADAM-TS 4; ADAMTS-2; ADAMTS4; ADAM-TS4; ADAMTS-4; ADMP-1; ADMP-1EC 3.4.24.82; Aggrecanase 1; aggrecanase-1; EC 3.4.24; KIAA0688A disintegrin and metalloproteinase with thrombospondin motifs 4