Human ADAMTS5 Antibody

Catalog #: MAB21981 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB21981-100
MAB21981-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ADAMTS5 Antibody Summary

Species Reactivity

Human

Specificity

Detects human ADAMTS5 in direct ELISA.

Source

Monoclonal Mouse IgG₁ Clone # 362821

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human ADAMTS5
Ser262-Pro622
Accession # Q9UNA0

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human ADAMTS5 Monoclonal Antibody (Catalog # MAB2198).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human ADAMTS5 DuoSet ELISA Kit (Catalog # DY2198-05) for convenient development of a sandwich ELISA.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

ELISA

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Human ADAMTS5 ELISA Standard Curve. Recombinant Human ADAMTS5 protein was serially diluted 2-fold and captured by Mouse Anti-Human ADAMTS5 Monoclonal Antibody (Catalog # MAB2198) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human ADAMTS5 Monoclonal Antibody (Catalog # MAB21981) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ADAMTS5

ADAMTS5 (a disintegrin and metalloproteinase with thrombospondin motifs 5), also known as Aggrecanase-2 and ADAMTS11, is a member of the family of secreted zinc proteases with a multi-domain structure (1, 2). The protein precursors consist of signal peptide and following domains: pro, catalytic, disintegrin-like, TS type 1 motif, cysteine-rich, spacer and a variable number of TS type 1 motifs. ADAMTS5 is an active protease effectively cleaving alpha 2-Macroglobulin (3), Aggrecan (4), and Brevican (5), and is inhibited by TIMP-3 with inhibition constants in the subnanomolar range (6). Based on the murine model studies (7, 8), this protease may be a key enzyme in the degradation of cartilage leading to osteoarthritis and rheumatoid arthritis. The purified recombinant human (rh) ADAMTS5 starts at the N-terminus of the catalytic domain and ends at the C-terminus of the TSP-1 domain. The amino acid sequence of rhADAMTS5 is 98%, 97%, and 96% identical to that of canine, bovine, and mouse/rat. The aggrecanase activity can be inhibited by 5 mM 1,10-phenanthroline and rhTIMP-3 (R&D Systems, Catalog # 973-TM).

References

Porter, S. et al. (2005) Biochem. J. 386:15.
Nagase, H. and M. Kashiwagi (2003) Arthritis Res. Ther. 5:94.
Tortorella, M.D. et al. (2004) J. Biol. Chem. 279:17554.
Vankemmelbeke, M.N. et al. (2001) Eur. J. Biochem. 268:1259.
Nakada, M. et al. (2005) Acta Neurophathol (Berl). 110:239.
Kashiwagi, M. et al. (2001) J. Biol. Chem. 276:12501.
Glasson, S.S. et al. (2005) Nature, 434: 644.
Stanton, H. et al. (2005) Nature, 434:648.

Long Name

A Disintegrin-like and Metalloproteinase Domain with Thrombospondin Motifs 5

Entrez Gene IDs

11096 (Human); 23794 (Mouse); 304135 (Rat)

Alternate Names

A disintegrin and metalloproteinase with thrombospondin motifs 11; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondintype 1 motif, 5 (aggrecanase-2); ADAM metallopeptidase with thrombospondin type 1 motif, 5; ADAM-TS 11; ADAM-TS 5; ADAMTS11; ADAMTS-11; ADAMTS5; ADAM-TS5; ADAMTS-5; ADMP2; ADMP-2; ADMP-2A disintegrin and metalloproteinase with thrombospondin motifs 5; Aggrecanase 2; aggrecanase-2; disintegrin-like and metalloprotease with thrombospondin type 1 motif, 510ADAMTS11FLJ36738; EC 3.4.24; EC 3.4.24.-; EC 3.4.24.14; EC 3.4.24.82