Human ADAMTS5 Antibody

Catalog #: AF2198 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF2198
AF2198-SP

All ADAMTS5 Antibodies

Product Details

Supplemental Products

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ADAMTS5 Antibody Summary

Species Reactivity

Human

Specificity

Detects human ADAMTS5 in direct ELISAs and Western blots. In Western blots, less than 5% cross-reactivity with recombinant human (rh) ADAMTS1 and rhADAMTS-L1.2 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human ADAMTS5
Ser262-Pro622
Accession # Q9UNA0

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human ADAMTS5 (Catalog # 2198-AD)

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human ADAMTS5 (Catalog # 2198‑AD), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ADAMTS5

ADAMTS5 (a disintegrin and metalloproteinase with thrombospondin motifs 5), also known as aggrecanase-2 and ADAMTS11, is a member of the family of secreted zinc proteases with a multi-domain structure (1, 2). The protein precursors consist of signal peptide and following domains: pro, catalytic, disintegrin-like, TS type 1 motif, cysteine-rich, spacer and a variable number of TS type 1 motifs. ADAMTS5 is an active protease effectively cleaving alpha 2-macroglobulin (3), aggrecan (4), and brevican (5), and is inhibited by TIMP-3 with inhibition constants in the subnanomolar range (6). Based on the murine model studies (7, 8), this protease may be a key enzyme in the degradation of cartilage leading to osteoarthritis and recombinant human eumatoid arthritis. The purified recombinant human ADAMTS5 starts at the
N‑terminus of the catalytic domain and ends at the C-terminus of the TSP-1 domain. The amino acid sequence of recombinant human ADAMTS5 is 98%, 97%, and 96% identical to that of canine, bovine, and mouse/rat. The aggrecanase activity can be inhibited by 5 mM 1,10-phenanthroline and recombinant human TIMP-3 (Catalog # 973-TM).

References

Porter, S. et al. (2005) Biochem. J. 386:15.
Nagase, H. and M. Kashiwagi (2003) Arthritis Res. Ther. 5:94.
Tortorella, M.D. et al. (2004) J. Biol. Chem. 279:17554.
Vankemmelbeke, M.N. et al. (2001) Eur. J. Biochem. 268:1259.
Nakada, M. et al. (2005) Acta Neurophathol (Berl). 110:239.
Kashiwagi, M. et al. (2001) J. Biol. Chem. 276:12501.
Glasson, S.S. et al. (2005) Nature, 434: 644.
Stanton, H. et al. (2005) Nature, 434:648.

Long Name

A Disintegrin-like and Metalloproteinase Domain with Thrombospondin Motifs 5

Entrez Gene IDs

11096 (Human); 23794 (Mouse); 304135 (Rat)

Alternate Names

A disintegrin and metalloproteinase with thrombospondin motifs 11; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondintype 1 motif, 5 (aggrecanase-2); ADAM metallopeptidase with thrombospondin type 1 motif, 5; ADAM-TS 11; ADAM-TS 5; ADAMTS11; ADAMTS-11; ADAMTS5; ADAM-TS5; ADAMTS-5; ADMP2; ADMP-2; ADMP-2A disintegrin and metalloproteinase with thrombospondin motifs 5; Aggrecanase 2; aggrecanase-2; disintegrin-like and metalloprotease with thrombospondin type 1 motif, 510ADAMTS11FLJ36738; EC 3.4.24; EC 3.4.24.-; EC 3.4.24.14; EC 3.4.24.82