Human Adenosine A1R Antibody

Catalog #: MAB11135 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB11135-100
MAB11135-SP

Detection of Adenosine A1R in human liver cancer.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Adenosine A1R Antibody Summary

Species Reactivity

Human

Specificity

Detects human Adora-1 in direct and cell-based elisas

Source

Monoclonal Mouse IgG₁ Clone # 1051024

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Human Adora-1 synthetic peptide corresponding to the 2nd. extra-cellular loop.

Accession # P30542

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

3-25 µg/mL

Immersion fixed paraffin-embedded human liver cancer

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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Detection of Adenosine A1R in human liver cancer. Adenosine A1R was detected in immersion fixed paraffin-embedded sections of liver cancer using Mouse Anti-Human Adenosine A1R Monoclonal Antibody (Catalog # MAB11135) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with Hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Adenosine A1R

Adenosine A1 Receptor (ADORA1, Adenosine A1R) is an adenosine receptor that belongs to the G protein-coupled receptor family (GPCR). There are 3 types of adenosine receptors, each with a specific pattern of ligand binding and tissue distribution, and together they regulate a diverse set of physiologic functions. The Adenosine A1R receptor inhibits adenylyl cyclase, and plays a role in the fertilization process. Animal studies also suggest a role for A1 receptors in kidney function and ethanol intoxication.

Long Name

Adenosine A1 Receptor

Entrez Gene IDs

134 (Human); 11539 (Mouse); 29290 (Rat)

Alternate Names

Adenosine A1 R; adenosine A1 receptor; Adenosine A1R; ADORA 1; ADORA1; RDC7; RDC7adenosine receptor A1