Human Adenosine A2aR/A2bR Antibody

Catalog #: MAB94972 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB94972-100
MAB94972-SP

Detection of Adenosine A2aR in HEK293 Human Cell Line Transfected with Human Adenosine A2aR and eGFP by Flow Cytometry.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Adenosine A2aR/A2bR Antibody Summary

Species Reactivity

Human

Specificity

Detects human Adenosine A2aR and human Adenosine A2bR in flow cytometry.

Source

Monoclonal Mouse IgG_2B Clone # 599743

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human Adenosine A2aR
Met1-Ser412
Accession # P29274

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Adenosine A2aR in HEK293 Human Cell Line Transfected with Human Adenosine A2aR and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human Adenosene A2aR or (B) irrelevant protein and eGFP was stained with Mouse Anti-Human Adenosine A2aR/A2bR Monoclonal Antibody (Catalog # MAB94972) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry

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Detection of Adenosine A2bR in HEK293 Human Cell Line Transfected with Human Adenosine A2bR and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human Adenosene A2bR and eGFP was stained with (A) Mouse Anti-Human Adenosine A2aR/A2bR Monoclonal Antibody (Catalog # MAB94972) or (B) isotype control antibody (Catalog # MAB0041) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Adenosine A2aR/A2bR

Adenosine is a ubiquitous endogenous molecule that affects almost all aspects of cellular physiology. The four Adenosine G protein-coupled receptors (GPCR) A₁, A_2A, A_2B, and A₃ have been described as key metabolic and immune-checkpoint regulators implicated in the tumor escape from the host immune system becoming both, markers of pathologies, and useful targets for novel drugs. Adenosine receptors A_2A and A_2B (also known as ADORA2A and ADORA2B, respectively) have been also shown to play an important cardio-protective role.

Long Name

Adenosine A2A Receptor/Adenosine A2B Receptor

Alternate Names

Adenosine A2aR/A2bR