Human Adenosine A2aR/A2bR Antibody

Catalog #: MAB94973 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB94973-100
MAB94973-SP

Detection of Adenosine A2aR/A2bR in HEK293 cells transfected with Human ADORA-2A and eGFP cells by Flow Cytometry.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Adenosine A2aR/A2bR Antibody Summary

Species Reactivity

Human

Specificity

Detects human Adenosine A2aR in direct ELISA.

Source

Monoclonal Mouse IgG_2B Clone # 826037

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human Adenosine A2aR
Met1-Ser412
Accession # P29274

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

HEK293 cells transfected with Human ADORA-2A and eGFP

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Adenosine A2aR/A2bR in HEK293 cells transfected with Human ADORA-2A and eGFP cells by Flow Cytometry. HEK293 cells transfected with Human ADORA-2A and eGFP were stained with eGFP and either (A) Mouse Anti-Human Adenosine A2aR/A2bR Monoclonal Antibody (Catalog # MAB94973) or (B) Mouse IgG_2B Isotype Control (Catalog # MAB004) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Adenosine A2aR/A2bR

Adenosine is a ubiquitous endogenous molecule that affects almost all aspects of cellular physiology. The four Adenosine G protein-coupled receptors (GPCR) A₁, A_2A, A_2B, and A₃ have been described as key metabolic and immune-checkpoint regulators implicated in the tumor escape from the host immune system becoming both, markers of pathologies, and useful targets for novel drugs. Adenosine receptors A_2A and A_2B (also known as ADORA2A and ADORA2B, respectively) have been also shown to play an important cardio-protective role.

Long Name

Adenosine A2A Receptor/Adenosine A2B Receptor

Alternate Names

Adenosine A2aR/A2bR