Human Adenosine A2b R Antibody

Catalog #: MAB10444 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB10444-100
MAB10444-SP

Detection of Human Adenosine A2b R by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Adenosine A2b R Antibody Summary

Species Reactivity

Human

Specificity

Detects human Adenosine A2b R in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 1022311

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Synthetic peptide containing human Adenosine A2b R

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Adenosine A2b R by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line and human cerebeullum tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Adenosine A2b R Monoclonal Antibody (Catalog # MAB10444) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Adenosine A2b R at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Adenosine A2b R

ADORA2B (Adenosine B2a Receptor, Adora-B2) is a widely expressed seven transmembrane G protein-coupled receptor (GPCR) activated by adenosine. Studies show that adenosine is crucial for ischemic preconditioning (IP) via its receptors ADORA1, ADORA2a and ADORA2b. All of these receptors are expressed in the myocardium and have been implicated in the role of cardioprotection during ischemia. Human and mouse Adora2B sequences are 88% identical over the full protein, but only 77% identical on the extra-cellular domains, including N-term tail and three loops.

Long Name

Adenosine A2b Receptor

Entrez Gene IDs

136 (Human); 11541 (Mouse); 29316 (Rat)

Alternate Names

Adenosine A2b R; adenosine A2b receptor; Adenosine A2bR; adenosine receptor A2b; ADORA2; ADORA2B