Human Adenosine A2b R Antibody

Catalog #: MAB104442 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB104442-100
MAB104442-SP

Detection Adenosine A2b R in HEK293 Human Cell Line Transfected with Human Adenosine A2b R and eGFP by Flow Cytometry

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Adenosine A2b R Antibody Summary

Species Reactivity

Human

Specificity

Detects human Adenosine A2b R in direct ELISAs.

Source

Recombinant Monoclonal Rabbit IgG Clone # 2683F

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Human Adenosine A2b R synthetic peptide
Accession # NP_000667

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

HEK293 Human Cell Line Transfected with Human Adenosine A2b R and eGFP

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection Adenosine A2b R in HEK293 Human Cell Line Transfected with Human Adenosine A2b R and eGFP by Flow Cytometry HEK293 human embryonic kidney cell line transfected with either (A) human Adenosine A2b R or (B) irrelevant protein and eGFP was stained with Rabbit Anti-Human Adenosine A2b R Monoclonal Antibody (Catalog # MAB104442) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (F0111). Quadrant markers were set based on Rabbit IgG control antibody (MAB1050). Staining was performed using our Staining Membrane-associated Proteins protocol.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Adenosine A2b R

ADORA2B (Adenosine B2a Receptor, Adora-B2) is a widely expressed seven transmembrane G protein-coupled receptor (GPCR) activated by adenosine. Studies show that adenosine is crucial for ischemic preconditioning (IP) via its receptors ADORA1, ADORA2a and ADORA2b. All of these receptors are expressed in the myocardium and have been implicated in the role of cardioprotection during ischemia. Human and mouse Adora2B sequences are 88% identical over the full protein, but only 77% identical on the extra-cellular domains, including N-term tail and three loops.

Long Name

Adenosine A2b Receptor

Entrez Gene IDs

136 (Human); 11541 (Mouse); 29316 (Rat)

Alternate Names

Adenosine A2b R; adenosine A2b receptor; Adenosine A2bR; adenosine receptor A2b; ADORA2; ADORA2B