Human Adenosylhomocysteinase/AHCY Antibody Summary
Ser2-Tyr432
Accession # P23526
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human Adenosylhomocysteinase/AHCY by Western Blot. Western blot shows lysates of Bowes human melanoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Human Adenosylhomocysteinase/AHCY Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6466) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Adenosylhomocysteinase/AHCY at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
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Detection of Human Adenosylhomocysteinase/AHCY by Simple WesternTM. Simple Western shows lysates of Exosome Standards (HT‑29) (NBP3-11685), Exosome Standards (PC‑3) (NBP2-49856) and K562 human chronic myelogenous leukemia cell line, loaded at 0.5 mg/ml. A specific band was detected for Adenosylhomocysteinase/AHCY at approximately 50 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human Adenosylhomocysteinase/AHCY Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6466). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Adenosylhomocysteinase/AHCY
Human S‑Adenosylhomocysteinase (AHCY) is a cytoplasmic tetramer with a tightly bound NAD co‑factor for each subunit (1, 2). It is the only known enzyme to catalyze the breakdown of S‑adenosylhomocysteine (AdoHcy) to homocysteine and adenosine. AdoHcy hydrolysis is a reversible reaction with an equilibrium favoring AdoHcy formation, but hydrolysis prevails under physiological conditions due to the rapid removal of adenosine and homocysteine. Thus, AHCY’s activity in mammals is directly related to homocysteine level, an independent risk factor for vascular disease (3). It also functions as a regulator of biological transmethylation by controlling the concentration of AdoHcy, a potent competitive inhibitor of all S‑adonosyl-L-methionine methyltransferases (1). A mutation in the human AHCY results in AHCY deficiency with increase of plasma creatine kinase, methionine, S‑adenosylmethionine and AdoHcy, delay of myelination, myopathy and psychomotor retardation (4, 5).
- Turner, M. A. et al. (2000) Cell Biochem. Biophys. 33:101.
- Takata, Y. et al. (2002) J. Biol. Chem. 277:22670.
- Gellekink, H. et al. (2004) Eur. J. Hum. Genet. 12:942.
- Baric, I. et al. (2004) Proc. Natl. Acad. Sci. USA. 101:4234.
- Fumic, K. et al. (2007) Eur. J.Hum. Genet. 15:347.
Product Datasheets
Citation for Human Adenosylhomocysteinase/AHCY Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Inhibition of cellular methyltransferases promotes endothelial cell activation by suppressing glutathione peroxidase 1 protein expression.
Authors: Barroso M, Florindo C, Kalwa H, Silva Z, Turanov A, Carlson B, de Almeida I, Blom H, Gladyshev V, Hatfield D, Michel T, Castro R, Loscalzo J, Handy D
J Biol Chem, 2014-04-09;289(22):15350-62.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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