Human Adiponutrin/PNPLA3 Antibody

Catalog # Availability Size / Price Qty
MAB5208-100
MAB5208-SP
Detection of Adiponutrin/PNPLA3 in Human Kidney.
1 Image
Product Details
FAQs
Supplemental Products
Reviews

Human Adiponutrin/PNPLA3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Adiponutrin/PNPLA3 in direct ELISA.
Source
Monoclonal Mouse IgG2B Clone # 847516
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Adiponutrin/PNPLA3
Arg160-Arg349
Accession # Q9NST1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
5-25 µg/mL
Immersion fixed paraffin-embedded sections of Human Kidney

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry View Larger

Detection of Adiponutrin/PNPLA3 in Human Kidney. Adiponutrin/PNPLA3 was detected in immersion fixed paraffin-embedded sections of Human Kidney using Mouse Anti-Human Adiponutrin/PNPLA3 Monoclonal Antibody (Catalog # MAB5208) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epitehelial cells in convoluted tubules. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Loading...
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Adiponutrin/PNPLA3

Human Adiponutrin (ADPN; also PNPLA3 and patatin-like phospholipase domain-containing protein 3) is a 53 kDa member of the PNPLA family of phospholipase A2 enzymes. It is expressed in white adipocytes. Adiponutrin is elevated in response to insulin and appears to act not as a phospholipase, but a molecule involved in anabolic pathways. ADPN is likely to be a type II transmembrane protein that is 481 amino acids (aa) in length. It contains one patatin domain (aa 10‑179) that is associated with in vitro enzyme activity and a transmembrane segment between aa 42‑62. There appear to be two splice variants. One shows a four aa deletion of aa 59‑62 and a second shows an alternate start site at Met379. Over aa 160‑349, human ADPN shares 61% aa identity with mouse Adiponutrin.

Long Name
Patanin-like Phospholipase Domain-containing Protein 3
Entrez Gene IDs
80339 (Human); 362972 (Rat)
Alternate Names
Acylglycerol O-acyltransferase; Adiponutrin; ADPN; ADPNdJ796I17.1; C22orf20; Calcium-independent phospholipase A2-epsilon; chromosome 22 open reading frame 20; EC 2.3.1.-; EC 2.7.7.56; EC 3.1.1.3; EC 4.2.3.4; FLJ22012; iPLA(2)epsilon; iPLA2-epsilon; patatin-like phospholipase domain containing 3; patatin-like phospholipase domain-containing protein 3; PNPLA3

Product Datasheets

You must select a language.

x

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human Adiponutrin/PNPLA3 Antibody

There are currently no reviews for this product. Be the first to review Human Adiponutrin/PNPLA3 Antibody and earn rewards!

Have you used Human Adiponutrin/PNPLA3 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review