Human ADP-Sugar Pyrophosphatase/NUDT5 Antibody

Catalog #: MAB6414 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6414
MAB6414-SP

Detection of Human ADP‑Sugar Pyrophosphatase/NUDT5 by Western Blot.

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All ADP-Sugar Pyrophosphatase/NUDT5 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ADP-Sugar Pyrophosphatase/NUDT5 Antibody Summary

Species Reactivity

Human

Specificity

Detects human ADP-Sugar Pyrophosphatase/NUDT5 in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 739044

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human ADP-Sugar Pyrophosphatase/NUDT5
Glu2-Phe219
Accession # Q9UKK9

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human ADP‑Sugar Pyrophosphatase/NUDT5 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and human liver tissue. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human ADP-Sugar Pyrophosphatase/NUDT5 Monoclonal Antibody (Catalog # MAB6414) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ADP-Sugar Pyrophosphatase/NUDT5 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ADP-Sugar Pyrophosphatase/NUDT5

ADP-Sugar Pyrophosphatase (NUDT5) is a member of the nudix superfamily of enzymes. Members of this family are pyrophosphohydrolases that act upon substrates with the general structure of a nucleoside (Nu) diphosphate (di) linked to another moiety, X (NDP-X) to yield NMP plus P-X (1). Human NUDT5 is a homodimeric enzyme present in the cytosol of most cell types (2). Glu166 and three magnesium ions are important for stabilizing the transition state during the hydrolysis of ADPR (3). NUDT5 has been suggested to play a role in regulating the intracellular levels of ADPR by NO activation through ADP-ribosylation at cysteine residues of the enzyme in macrophages (4). It also may play defensive role against the mutagenesis induced by oxidized deoxyribonucleosides (5, 6).

References

McLennan, A.G. (2006) Cell Mol. Life Sci. 63:123.
Zha, M. et al. (2006) J. Mol. Biol. 364:1021.
Zha, M. et al. (2008) J. Mol. Biol. 379:568.
Yu, H.N. et al. (2007) Biochem. Biophys. Res. Comm. 354:764.
Hori, M. et al. (2010) Free Radic. Biol. Med. 48:1197.
Kamiya, H. et al. (2009) DNA Repair (Amst) 8:1250.

Entrez Gene IDs

11164 (Human)

Alternate Names

EC 3.6.1.-; EC 3.6.1.13; hYSAH1; nucleoside diphosphate linked moiety X-type motif 5; Nucleoside diphosphate-linked moiety X motif 5; nudix (nucleoside diphosphate linked moiety X)-type motif 5; Nudix motif 5; NUDT5; YSA1; YSA1H; YSA1HADP-sugar pyrophosphatase