Human AGTR-1 Antibody

Catalog #: MAB10244 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB10244-100
MAB10244-SP

Detection of AGTR-1 in HEK293 Human Cell Line Transfected with Human AGTR-1 and eGFP by Flow Cytometry.

1 Image

All AGTR-1 Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human AGTR-1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human AGTR-1 in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 1010103

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Synthetic peptide containing human AGTR-1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

View Larger

Detection of AGTR-1 in HEK293 Human Cell Line Transfected with Human AGTR-1 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) Human AGTR-1 or (B) irrelevant protein, and EGFP, was stained with Mouse Anti-Human AGTR-1 Monoclonal Antibody (Catalog # MAB10244) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). Quadrant markers were set based on Mouse IgG2B isotype control antibody staining (MAB0041). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: AGTR-1

Type-1 angiotensin II receptor, AGTR1, is a seven transmembrane domain (7TM) G protein-coupled receptor (GPCR) that mediates the cardiovascular effects of angiotensin II. At least four transcript variants have been described for the AGTR1 gene.

Long Name

Angiotensin II Receptor Type 1

Entrez Gene IDs

185 (Human); 11607 (Mouse); 24180 (Rat)

Alternate Names

AG2S; AGTR1; AGTR-1; AGTR1AAngiotensin II type-1 receptor; Angiostensin Receptor; angiotensin II receptor, type 1; angiotensin receptor 1; angiotensin receptor 1B; AT1; AT1AR; AT1AT1AR; AT1B; AT1BR; AT1R; AT2R1; AT2R1A; AT2R1AGTR1B; AT2R1BAT1BR; HAT1R; type-1 angiotensin II receptor; type-1B angiotensin II receptor