Home / Airway Trypsin-like Protease/HAT / Human Airway Trypsin-like Protease/HAT Antibody

Human Airway Trypsin-like Protease/HAT Antibody

Catalog #: MAB2695
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB2695
MAB2695-SP
Product Details
Citations (2)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Airway Trypsin-like Protease/HAT Antibody Summary

Species Reactivity
Human
Specificity
Detects human Airway Trypsin-like Protease/HAT in direct ELISAs and Western blots. Recognizes the catalytic-domain of recombinant human HAT.
Source
Monoclonal Mouse IgG1 Clone # 337029
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Airway Trypsin-like Protease/HAT
Ala42-Ile418
Accession # O60235
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Airway Trypsin-like Protease/HAT (Catalog # 2695-SE)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Airway Trypsin-like Protease/HAT

Human Airway Trypsin‑like protease (HAT), also known as Transmembrane Protease, Serine 11D (TMPRSSIID), is a type II transmembrane serine protease that is expressed in the cells of the submucosal serous glands of the bronchi and trachea (1‑3). The deduced sequence predicts a short cytoplasmic tail (aa 120), a transmembrane domain (aa 21‑41), and an ectodomain consisting of a SEA domain (aa 44‑164) and a catalytic domain (aa 187‑418). The single‑chain precursor can be converted into a disulfide‑linked two‑chains, one of which corresponds to the catalytic domain. HAT was initially purified from the sputum of patients with chronic airway diseases (1). HAT has been shown to induce PAR‑2 mediated IL‑8 release in psoriasis vulgaris and increase mucin expression in airway epithelial cells (4, 5). The amino acid sequence of human HAT is 99%, 79%, and 67% identical to that of chimpanzee, canine and rat/mouse.

References
  1. Yasuoka, S. et al. (1997) Am. J. Respir. Cell Mol. Biol. 16:300.
  2. Yamaoka, K. et al. (1998) J. Biol. Chem. 273:11895.
  3. Hooper, J.D. et al. (2001) J. Biol. Chem. 276:857.
  4. Iwakiri, K. et al. (2004) J. Invest. Dermatol. 122:937.
  5. Chokki, M. et al. (2004) Am. J. Respir. Cell Mol. Biol. 30:470.
Long Name
Human Airway Trypsin-like Protease
Entrez Gene IDs
9407 (Human); 231382 (Mouse)
Alternate Names
airway trypsin like protease; Airway trypsin-like protease; EC 3.4.21; EC 3.4.21.-; HAT; MGC150587; MGC150588; TMPRSS11D; transmembrane protease serine 11D; transmembrane protease, serine 11D

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Citations for Human Airway Trypsin-like Protease/HAT Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Nasal symbiont Staphylococcus epidermidis restricts the cellular entry of influenza virus into the nasal epithelium
    Authors: Ara Jo, Jina Won, Chan Hee Gil, Su Keun Kim, Kang-Mu Lee, Sang Sun Yoon et al.
    npj Biofilms and Microbiomes
  2. Influenza and SARS-coronavirus activating proteases TMPRSS2 and HAT are expressed at multiple sites in human respiratory and gastrointestinal tracts.
    Authors: Bertram S, Heurich A, Lavender H, Gierer S, Danisch S, Perin P, Lucas JM, Nelson PS, Pohlmann S, Soilleux EJ
    PLoS ONE, 2012-04-30;7(4):e35876.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P

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