Human ALK-7 Antibody

Name: Human ALK-7 Antibody
Brand: R&D Systems
SKU: MAB7749
Rating: 5 (1 reviews)

Catalog #: MAB7749 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB7749
MAB7749-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ALK-7 Antibody Summary

Species Reactivity

Human

Specificity

Detects human ALK‑7 in direct ELISAs. In direct ELISAs, 100% cross-reactivity with recombinant rat ALK7 is observed and no cross‑reactivity with recombinant mouse Activin RIA/ALK2 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 810510

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human ALK‑7
Leu26-Glu113
Accession # Q8NER5

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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ALK‑7 in Human Pancreas. ALK-7 was detected in immersion fixed paraffin-embedded sections of human pancreas using Mouse Anti-Human ALK-7 Monoclonal Antibody (Catalog # MAB7749) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter-stained with hematoxylin (blue). Specific staining was localized to islet cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ALK-7

Activin receptor-like kinase 7 (ALK-7), also known as Activin R1C (gene name ACVR1C), is a glycosylated 58 kDa type I receptor in the superfamily of TGF-beta serine/threonine kinase receptors. It associates with type II receptors to form a signaling complex that responds to the ligands Activin AB, and Activin B, GDF3, and Nodal. ALK-7 plays a role in regulating energy balance by inhibiting insulin secretion and inducing pancreatic beta cell apoptosis. It is expressed in adipose tissue but downregulated in obesity. ALK-7 is also expressed in pituitary gonadotropic cells and in pre-eclamptic placenta. It induces the apoptosis of trophoblasts as well as ovarian granulosa and epithelial cells. Within the extracellular domain, human ALK-7 shares 95% and 91% amino acid (aa) sequence identity with mouse and rat ALK-7, respectively. Alternate splicing of human ALK-7 generates additional isoforms with either a 50 aa N-terminal truncation or with deletions of 79 aa or 157 aa that encompass the transmembrane segment.

Long Name

Activin Receptor-like Kinase 7

Entrez Gene IDs

130399 (Human); 245921 (Rat)

Alternate Names

activin A receptor, type IC; Activin receptor type IC; activin receptor type-1C; Activin receptor-like kinase 7; Activin RIC; ACTR-IC; ACVR1C; ACVRLK7; ALK7; ALK-7; ALK7ALK-7; EC 2.7.11; EC 2.7.11.30