Human Alkaline Phosphatase/ALPL PE-conjugated Antibody

Catalog #: FAB1448P
15 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB1448P-100
FAB1448P-025

Detection of Alkaline Phosphatase/ALPL in BG01V Human Cells by Flow Cytometry.

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Citations (15)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Alkaline Phosphatase/ALPL PE-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects liver, bone and kidney Alkaline Phosphatase/ALPL from human tissue (2).

Source

Monoclonal Mouse IgG₁ Clone # B4-78

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human liver, bone and kidney-derived Alkaline Phosphatase/ALPL

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Alkaline Phosphatase/ALPL in BG01V Human Cells by Flow Cytometry. BG01V human embryonic stem cells were stained with Mouse Anti-Human Alkaline Phosphatase/ALPL PE-conjugated Monoclonal Antibody (Catalog # FAB1448P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Alkaline Phosphatase/ALPL

The liver, bone and kidney Alkaline Phosphatase, also known as tissue non-specific Alkaline Phosphatase, is a glycosyl phosphatidylinositol (GPI) anchored protein. Human liver/bone/kidney Alkaline Phosphatase shares 90% amino acid sequence homology with the mouse enzyme.

References

Lawson, G.M. et al. (1985) Clin. Chem. 31:381.
Gronthos, S. et al. (1999) J. Bone Miner. Res. 14:47.
Dorheim, M.A. et al. (1993) J. Cell Physiol. 154:317.

Long Name

Alkaline Phosphatase Liver

Entrez Gene IDs

249 (Human); 11647 (Mouse)

Alternate Names

Akp2; Alkaline phosphatase liver/bone/kidney isozyme; alkaline phosphatase, liver/bone/kidney; alkaline phosphatase, tissue-nonspecific isozyme; alkaline phosphomonoesterase; ALPL; APTNAP; AP-TNAP; EC 3.1.3.1; FLJ40094; FLJ93059; glycerophosphatase; HOPS; liver/bone/kidney-type alkaline phosphatase; MGC161443; tissue-nonspecific ALP; TNAP; TNSALP; TNSALPMGC167935

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human Alkaline Phosphatase/ALPL PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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Human osteoblasts obtained from distinct periarticular sites demonstrate differences in biological function in vitro
Authors: Erden Ali, Mark Birch, Niina Hopper, Neil Rushton, Andrew W. McCaskie, Roger A. Brooks
Bone & Joint Research
Osteogenic potential of adipogenic predifferentiated human bone marrow‐derived multipotent stromal cells for bone tissue‐engineering
Authors: Adrien Moya, Nathanaël Larochette, Marianne Bourguignon, Hanane El‐Hafci, Esther Potier, Hervé Petite et al.
Journal of Tissue Engineering and Regenerative Medicine
Single cell gene expression profiling of cortical osteoblast lineage cells
Authors: James M. Flynn, Steven C. Spusta, Clifford J. Rosen, Simon Melov
Bone
Botulinum Toxin Treatment of Adult Muscle Stem Cells from Children with Cerebral Palsy and hiPSC-Derived Neuromuscular Junctions
Authors: Costamagna, D;Bastianini, V;Corvelyn, M;Duelen, R;Deschrevel, J;De Beukelaer, N;De Houwer, H;Sampaolesi, M;Gayan-Ramirez, G;Campenhout, AV;Desloovere, K;
Cells
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Muscle Microbiopsy to Delineate Stem Cell Involvement in Young Patients: A Novel Approach for Children With Cerebral Palsy
Authors: M Corvelyn, N De Beukela, R Duelen, J Deschrevel, A Van Campen, S Prinsen, G Gayan-Rami, K Maes, G Weide, K Desloovere, M Sampaolesi, D Costamagna
Front Physiol, 2020-08-06;11(0):945.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Interstitial Cell Remodeling Promotes Aberrant Adipogenesis in Dystrophic Muscles
Authors: J Camps, N Breuls, A Sifrim, N Giarratana, M Corvelyn, L Danti, H Grosemans, S Vanuytven, I Thiry, M Belicchi, M Meregalli, K Platko, ME MacDonald, RC Austin, R Gijsbers, G Cossu, Y Torrente, T Voet, M Sampaolesi
Cell Rep, 2020-05-05;31(5):107597.
Species: Human
Sample Types: Whole Cells
Intrinsic Myogenic Potential of Skeletal Muscle-Derived Pericytes from Patients with Myotonic Dystrophy Type 1
Authors: CRM Ausems, RHL Raaijmaker, WJAA van den Br, M Willemse, BGM van Engele, DG Wansink, H van Bokhov
Mol Ther Methods Clin Dev, 2019-09-12;15(0):120-132.
Species: Human, Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
Alkaline Phosphatase Controls Lineage Switching of Mesenchymal Stem Cells by Regulating the LRP6/GSK3? Complex in Hypophosphatasia
Authors: W Liu, L Zhang, K Xuan, C Hu, L Li, Y Zhang, F Jin, Y Jin
Theranostics, 2018-11-09;8(20):5575-5592.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Fate of mesoangioblasts in a vaginal birth injury model: influence of the route of administration
Authors: MGMC Mori da Cu, G Giacomazzi, G Callewaert, L Hympanova, F Russo, G Vande Veld, R Gijsbers, M Albersen, M Sampaolesi, J Deprest
Sci Rep, 2018-07-13;8(1):10604.
Species: Rat
Sample Types: Whole Cells
Applications: ICC
A novel strategy for enrichment and isolation of osteoprogenitor cells from induced pluripotent stem cells based on surface marker combination.
Authors: Ochiai-Shino H, Kato H, Sawada T, Onodera S, Saito A, Takato T, Shibahara T, Muramatsu T, Azuma T
PLoS ONE, 2014-06-09;9(6):e99534.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Leukocyte alkaline phosphatase (LAP) by flow cytometry.
Authors: Eshel, Elizabet, Sharabi-Nov, Adi, Dally, Najib
MLO Med Lab Obs, 2013-08-01;45(8):22, 24, 26.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Brief report: benchmarking human pluripotent stem cell markers during differentiation into the three germ layers unveils a striking heterogeneity: all markers are not equal.
Stem Cells, 2011-09-01;29(9):1469-74.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Stem cells from deciduous tooth repair mandibular defect in swine.
Authors: Zheng Y, Liu Y, Zhang CM
J. Dent. Res., 2009-03-01;88(3):249-54.
Species: Porcine
Sample Types: Whole Cells
Applications: Flow Cytometry

Mesodermal iPSC–derived progenitor cells functionally regenerate cardiac and skeletal muscle
Authors: Mattia Quattrocelli, Melissa Swinnen, Giorgia Giacomazzi, Jordi Camps, Ines Barthélemy, Gabriele Ceccarelli et al.
Journal of Clinical Investigation

Attenuating iPSC reprogramming stress with dominant-negative BET peptides
Authors: Md Emon Hossain, Ricardo Raul Cevallos, Ruowen Zhang, Kejin Hu
iScience