Human Alkaline Phosphatase/ALPL PerCP-conjugated Antibody

Name: Human Alkaline Phosphatase/ALPL PerCP-conjugated Antibody
Brand: R&D Systems
SKU: FAB1448C

Catalog #: FAB1448C Datasheet / COA / SDS

Discontinued Product

FAB1448C has been discontinued.

View all Alkaline Phosphatase/ALPL products.

Detection of Alkaline Phosphatase/ALPL in BG01V Human Cells by Flow Cytometry.

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Human Alkaline Phosphatase/ALPL PerCP-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects liver, bone and kidney Alkaline Phosphatase/ALPL from human tissue (2).

Source

Monoclonal Mouse IgG₁ Clone # B4-78

Purification

Protein A or G purified from ascites

Immunogen

Human liver, bone and kidney-derived Alkaline Phosphatase/ALPL

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

PerCP (Peridinin-chlorophyll Protein Complex) (Excitation= 482 and 564 nm, Emission= 675 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Alkaline Phosphatase/ALPL in BG01V Human Cells by Flow Cytometry. BG01V human embryonic stem cells were stained with Mouse Anti-Human Alkaline Phosphatase/ALPL PerCP-conjugated Monoclonal Antibody (Catalog # FAB1448C, filled histogram) or isotype control antibody (Catalog # IC002C, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Alkaline Phosphatase/ALPL

The liver, bone and kidney Alkaline Phosphatase, also known as tissue non-specific Alkaline Phosphatase, is a glycosyl phosphatidylinositol (GPI) anchored protein. Human liver/bone/kidney Alkaline Phosphatase shares 90% amino acid sequence homology with the mouse enzyme.

References

Lawson, G.M. et al. (1985) Clin. Chem. 31:381.
Gronthos, S. et al. (1999) J. Bone Miner. Res. 14:47.
Dorheim, M.A. et al. (1993) J. Cell Physiol. 154:317.

Long Name

Alkaline Phosphatase Liver

Entrez Gene IDs

249 (Human); 11647 (Mouse)

Alternate Names

Akp2; Alkaline phosphatase liver/bone/kidney isozyme; alkaline phosphatase, liver/bone/kidney; alkaline phosphatase, tissue-nonspecific isozyme; alkaline phosphomonoesterase; ALPL; APTNAP; AP-TNAP; EC 3.1.3.1; FLJ40094; FLJ93059; glycerophosphatase; HOPS; liver/bone/kidney-type alkaline phosphatase; MGC161443; tissue-nonspecific ALP; TNAP; TNSALP; TNSALPMGC167935