Human alpha -1 Adrenergic R/ADRA1A Antibody

Catalog #: MAB10298 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB10298-100
MAB10298-SP

Detection of Human alpha ‑1 Adrenergic R/ADRA1A by Western Blot.

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All alpha-1A Adrenergic R/ADRA1A Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human alpha -1 Adrenergic R/ADRA1A Antibody Summary

Species Reactivity

Human

Specificity

Detects human alpha ‑1 Adrenergic R/ADRA1A in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 518508

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

HEK293 human embryonic kidney cell line transfected with human alpha ‑1 Adrenergic R/ADRA1A
Accession # P35348

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Flow Cytometry

0.25 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human alpha ‑1 Adrenergic R/ADRA1A by Western Blot. Western blot shows lysates of HEK293T human embryonic kidney cell line either mock transfected or transfected with human a-1 Adrenergic R/ADRA1A. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human a-1 Adrenergic R/ADRA1A Monoclonal Antibody (Catalog # MAB10298) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for a-1 Adrenergic R/ADRA1A at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry

Detection of a-1 Adrenergic R/ADRA1A antibody in HEK293 Human Cell Line Transfected with Human a-1 Adrenergic R/ADRA1A and eGFP antibody by Flow Cytometry.

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Detection of alpha ‑1 Adrenergic R/ADRA1A in HEK293 Human Cell Line Transfected with Human alpha ‑1 Adrenergic R/ADRA1A and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human a-1 Adrenergic R/ADRA1A or (B) irrelevant protein and eGFP was stained with Mouse Anti-Human a-1 Adrenergic R/ADRA1A Monoclonal Antibody (Catalog # MAB10298) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: alpha-1A Adrenergic R/ADRA1A

ADRA1A (formerly known as alpha 1-C adrenergic receptor) is an alpha-adrenergic receptor which is mediated by G(q) and G(11) proteins. Alpha-1 Adrenoreceptors play an important role in mediating human prostatic smooth muscle contraction. Antagonists of ADRA1A include tamsulosin and silodosin which treat benign prostatic hyperplasia.

Long Name

Alpha-1A Adrenergic Receptor

Entrez Gene IDs

148 (Human); 1549 (Mouse); 29412 (Rat)

Alternate Names

a-1A AdrenergicR; ADRA1A; ADRA1Cadrenergic, alpha-1A-, receptor variant 1; ADRA1L1; adrenergic, alpha -1A-, receptor; adrenergic, alpha-1A-, receptor variant 3; adrenergic, alpha-1A-, receptor variant 5; adrenergic, alpha-1A-, receptor variant 8; adrenergic, alpha-1A-, receptor; adrenergic, alpha-1C-, receptor; alpha-1A Adrenergic R; alpha-1A adrenergic receptor; alpha1A AdrenergicR; Alpha-1A adrenoceptor; Alpha-1A adrenoreceptor; ALPHA1AAR; Alpha-1C adrenergic receptor; Alpha-adrenergic receptor 1c; G protein coupled receptor