Human alpha ₂-Macroglobulin Antibody

Catalog #: MAB19381 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB19381-100
MAB19381-SP

Human alpha 2‑Macroglobulin ELISA Standard Curve.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human alpha ₂-Macroglobulin Antibody Summary

Species Reactivity

Human

Specificity

Detects human alpha ₂-Macroglobulin in direct ELISA, this antibody does not cross-react with rhC5a or rmC5a.

Source

Monoclonal Mouse IgG₁ Clone # 257303

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human plasma-derived alpha ₂-Macroglobulin

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA capture antibody when paired with Goat Anti-Human alpha ₂‑Macroglobulin Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1938).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human alpha 2-Macroglobulin DuoSet ELISA Kit (Catalog # DY1938) for convenient development of a sandwich ELISA.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

ELISA

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Human alpha ₂‑Macroglobulin ELISA Standard Curve. Recombinant Human alpha ₂‑Macroglobulin protein was serially diluted 2-fold and captured by Mouse Anti-Human alpha ₂‑Macroglobulin Monoclonal Antibody (Catalog # MAB19381) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human alpha ₂‑Macroglobulin Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1938) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: alpha 2-Macroglobulin

Alpha 2-macroglobulin (A2M) is a general and irreversible protease inhibitor implicated in many processes. It is able to inhibit all four classes of proteases by a unique trapping mechanism.

Entrez Gene IDs

2 (Human)

Alternate Names

A2M; alpha 2Macroglobulin; alpha 2-Macroglobulin; alpha-2-M; alpha-2-macroglobulin; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5; CPAMD5; CPAMD5DKFZp779B086; FWP007; S863-7