Human alpha -2A Adrenergic R/ADRA2A Antibody

Catalog #: MAB10129 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB10129-100
MAB10129-SP

Detection of ADRA2A in HEK293 Human Cell Line Transfected with Human ADRA2A and eGFP by Flow Cytometry.

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All alpha-2A Adrenergic R/ADRA2A Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human alpha -2A Adrenergic R/ADRA2A Antibody Summary

Species Reactivity

Human

Specificity

Detects human alpha ‑2A Adrenergic R/ADRA2A in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 1006614

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Synthetic peptide containing Human alpha ‑2A Adrenergic R/ADRA2A

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of ADRA2A in HEK293 Human Cell Line Transfected with Human ADRA2A and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human ADRA2A or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human ADRA2A Monoclonal Antibody (Catalog # MAB10129) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: alpha-2A Adrenergic R/ADRA2A

Alpha-2-adrenergic receptors, including ADRA2A, are members of the G protein-coupled receptor superfamily. They include 3 highly homologous subtypes: alpha2A, alpha2B, and alpha2C. These receptors have a critical role in regulating neurotransmitter release from sympathetic nerves and from adrenergic neurons in the central nervous system. Studies in mouse revealed that both ADRA2A and ADRA2C subtypes were required for normal presynaptic control of transmitter release from sympathetic nerves in the heart and from central noradrenergic neurons. The ADRA2A subtype inhibited transmitter release at high stimulation frequencies, whereas the ADRA2C subtype modulated neurotransmission at lower levels of nerve activity.

Long Name

Alpha-2A Adrenergic Receptor

Entrez Gene IDs

150 (Human); 11551 (Mouse); 25083 (Rat)

Alternate Names

a-2A AdrenergicR; ADRA2; ADRA2A; ADRA2Ralpha-2-adrenergic receptor, platelet type; ADRARZNF32; adrenergic, alpha-2A-, receptor; Alpha-2 adrenergic receptor subtype C10; alpha-2A Adrenergic R; alpha-2A adrenergic receptor; alpha2A AdrenergicR; Alpha-2A adrenoceptor; Alpha-2A adrenoreceptor; alpha-2AAR subtype C10; ALPHA2AAR; Alpha-2AAR; ZNF32