Human alpha -Defensin 5 Antibody

Catalog #: MAB10248 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB10248-100
MAB10248-SP

Human alpha -Defensin 5 ELISA Standard Curve.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human alpha -Defensin 5 Antibody Summary

Species Reactivity

Human

Specificity

Detects human alpha -Defensin 5 in direct ELISAs.

Source

Recombinant Monoclonal Mouse IgG₁ Clone # 972207

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human alpha -Defensin 5
Thr64-Arg94
Accession # Q01523

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Human alpha -Defensin 5 Monoclonal Antibody (Catalog # MAB102481).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

ELISA

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Human alpha -Defensin 5 ELISA Standard Curve. Recombinant Human a-Defensin 5 protein was serially diluted 2-fold and captured by Mouse Anti-Human a-Defensin 5 Monoclonal Antibody (Catalog # MAB10248) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human a-Defensin 5 Monoclonal Antibody (Catalog # MAB102481) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: alpha-Defensin 5

Defensin alpha 5 (DEFA5), also known as human alpha defensin 5 (HD5), is a secreted antimicrobial protein encoded by the DEFA5 gene and a member of the Defensins family. Defensins are thought to kill microbes by permeabilizing their plasma membrane. DEFA5 is expressed in Paneth cells, which are secretory epithelial cells in the small intestine. Unlike other defensins, DEFA5 is stored in secretory vesicles as a propeptide, and is processed into different peptides with different potency and localization.

Long Name

Defensin Alpha 5

Entrez Gene IDs

1670 (Human)

Alternate Names

alpha-Defensin 5; DEF5; DEF5defensin, alpha 5, preproprotein; DEFA5; defensin 5; Defensin alpha 5; Defensin, alpha 5; defensin, alpha 5, Paneth cell-specific; defensin-5; HD-5; MGC129728