Human alpha 1-Acid Glycoprotein Antibody

Detection of Human alpha 1‑Acid Glycoprotein by Western Blot. Western blot shows lysates of human liver tissue. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human a1-Acid Glycoprotein Monoclonal Antibody (Catalog # MAB3694) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for a1-Acid Glycoprotein at approximately 40-50 kDa (as indicated). This experiment was conducted under reducing and non-reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human alpha 1‑Acid Glycoprotein by Western Blot. Western blot shows lysates of human plasma. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human a1-Acid Glycoprotein Monoclonal Antibody (Catalog # MAB3694) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for a1-Acid Glycoprotein at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

alpha 1-Acid Glycoprotein in Human Liver. a1-Acid Glycoprotein was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human a1-Acid Glycoprotein Monoclonal Antibody (Catalog # MAB3694) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.