Human alpha 1-Acid Glycoprotein Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human alpha 1‑Acid Glycoprotein by Western Blot. Western blot shows human plasma. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human a1-Acid Glycoprotein Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3694) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for a1-Acid Glycoprotein at approximately 45-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of Human alpha 1‑Acid Glycoprotein by Simple WesternTM. Simple Western lane view shows lysates of human serum, loaded at 1:5000. A specific band was detected for a1-Acid Glycoprotein at approximately 79 kDa (as indicated) using 5 µg/mL of Goat Anti-Human a1-Acid Glycoprotein Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3694) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: alpha 1-Acid Glycoprotein
alpha 1-acid Glycoprotein, also known as orosomucoid-1, is a positive acute-phase reactant that is produced by the liver and secreted in plasma. Plasma alpha 1-acid concentration increases in response to inflammation and infection. alpha 1-acid glycoprotein belongs to the lipocalin family and binds numerous basic and neutral lipophilic drugs and steroid hormones. It is a 183 amino acid protein with 5 N-linked glycans that comprises 45% of the 43 kDa mass of the protein. The human and mouse protein share 43% amino acid sequence identity.
Product Datasheets
Citation for Human alpha 1-Acid Glycoprotein Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Astrocytic orosomucoid-2 modulates microglial activation and neuroinflammation
Authors: M Jo, JH Kim, G Song, M Seo, E Mi Hwang, K Suk
J. Neurosci, 2017-02-13;0(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
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