Human AMIGO Antibody

Catalog #: AF2565 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF2565
AF2565-SP

All AMIGO Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human AMIGO Antibody Summary

Species Reactivity

Human

Specificity

Detects human AMIGO in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) AMIGO2 is observed and less than 1% cross-reactivity with rhAMIGO3 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human AMIGO
Met1-Gly365
Accession # Q86WK6

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human AMIGO

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: AMIGO

AMIGO is a 65 kDa type I transmembrane glycoprotein that belongs to both the Ig- and LLR (leucine-rich repeat)- superfamilies. It is expressed on developing neuronal fiber tracts and participates in their formation. AMIGO forms both homophilic and heterophilic complexes with AMIGO2 and 3, suggesting an adhesive function for the molecule. Human AMIGO extracellular domain is 87% amino acid identical to mouse AMIGO, and 40% identical to human AMIGO2 and 3.

Long Name

Amphoterin-induced Gene and ORF

Entrez Gene IDs

57463 (Human); 229715 (Mouse)

Alternate Names

adhesion molecule with Ig-like domain 1; ali2; Alivin-2; AMIGO; AMIGO1; AMIGO-1; AMIGOamphoterin-induced protein 1; amphoterin-induced gene and ORF; KIAA1163