Human Amyloid beta (aa1-40) Quantikine ELISA Kit

Catalog #: DAB140B
19 Citations Datasheet / COA / SDS

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DAB140B

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QC216: Quantikine Immunoassay Control Set 1067 Human Amyloid beta

Human Amyloid beta (aa 1-40) ELISA Standard Curve

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All Amyloid beta ELISAs

Product Details

Citations (19)

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human Amyloid beta (aa1-40) Quantikine ELISA Kit Summary

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (200 uL), Tissue Lysates (10 uL), Cerebrospinal Fluid (10 uL)

Sensitivity

8.17 pg/mL

Assay Range

15.6 - 1,000 pg/mL (Cell Culture Supernates, Tissue Lysates, Cerebrospinal Fluid)

Specificity

Natural human Amyloid beta (aa1-40).

Cross-reactivity

Cross-reactivity observed with 1 or more available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

Interference observed with 1 or more available related molecules.

Product Summary

The Quantikine^® Human Amyloid beta (aa1-40) Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Amyloid beta (aa1-40) in cell culture supernates, tissue lysates, and cerebrospinal fluid. It contains synthetic human Amyloid beta (aa1-40) and antibodies raised against the synthetic peptide. Results obtained for naturally occurring human Amyloid beta (aa1-40) showed linear curves that were parallel to the standard curves obtained using the Quantikine^® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human Amyloid beta (aa1-40).

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

Cell Culture Supernates, Tissue Lysates, Cerebrospinal Fluid

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	58.9	264	556	56.3	247	518
Standard Deviation	4.23	5.76	11.3	4.31	22.4	30.5
CV%	7.2	2.2	2	7.7	9.1	5.9

Recovery

The recovery of human Amyloid beta spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	99	95-105
Cell Lysis Buffer (n=2)	99	91-110
Cerebrospinal Fluid (n=4)	104	96-113

Linearity

To assess linearity of the assay, samples containing and/or spiked with high concentrations of human Amyloid beta were diluted with Diluent RD2-7 to produce samples with values within the dynamic range of the assay.

Scientific Data

N/A

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Human Amyloid beta (aa 1-40) ELISA Standard Curve

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Human Amyloid beta (aa 1-40) ELISA Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Amyloid beta

Amyloid Precursor Protein (APP) is a type I transmembrane protein that is ubiquitously expressed on cell surfaces. It undergoes complex proteolytic processing and is cleaved by alpha-, beta-, and gamma-Secretases to generate soluble APP alpha, soluble APP beta, and Amyloid beta (A beta) fragments of several lengths. One of these fragments, A beta 42, generated by beta- and gamma-Secretase activities, has been implicated in Alzheimer's disease. Aberrantly high levels of this peptide form and accumulate in the brains of Alzheimer's disease patients to create the senile plaques characteristic of the disease.

Entrez Gene IDs:

351 (Human); 11820 (Mouse)

Alternate Names:

Abeta; Amyloid beta; beta-Amyloid Peptide

Related Research Areas

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Human Amyloid beta (aa1-40) Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

19 Citations: Showing 1 - 10
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Alzheimer's disease protective allele of Clusterin modulates neuronal excitability through lipid-droplet-mediated neuron-glia communication
Authors: Zhao, X;Li, Y;Zhang, S;Sudwarts, A;Zhang, H;Kozlova, A;Moulton, MJ;Goodman, LD;Pang, ZP;Sanders, AR;Bellen, HJ;Thinakaran, G;Duan, J;
medRxiv : the preprint server for health sciences
Species: Human
Sample Types: Cell Culture Supernates
Altered ubiquitin signaling induces Alzheimer's disease-like hallmarks in a three-dimensional human neural cell culture model
Authors: Maniv, I;Sarji, M;Bdarneh, A;Feldman, A;Ankawa, R;Koren, E;Magid-Gold, I;Reis, N;Soteriou, D;Salomon-Zimri, S;Lavy, T;Kesselman, E;Koifman, N;Kurz, T;Kleifeld, O;Michaelson, D;van Leeuwen, FW;Verheijen, BM;Fuchs, Y;Glickman, MH;
Nature communications
Species: Human
Sample Types: Cell Culture Supernates, Cell Lysates
MiR-431 attenuates synaptic plasticity and memory deficits in APPswe/PS1dE9 mice
Authors: Ge, J;Xue, Z;Shu, S;Yu, L;Qin, R;Tao, W;Liu, P;Dong, X;Lan, Z;Bao, X;Ye, L;Xu, Y;Zhu, X;
JCI insight
Species: Mouse
Sample Types: Tissue Homogenates
Machine-learning-based analysis of the sensitivity and specificity on lipid-lowering effect of one-month-administered statins
Authors: H Liu, R Jiao, L Wang, F Feng, X Zhao, J Yang
Medicine, 2023-03-03;102(9):e33139.
Species: Human
Sample Types: Serum
Human PSEN1 Mutant Glia Improve Spatial Learning and Memory in Aged Mice
Authors: H Jäntti, M Oksanen, P Kettunen, S Manta, L Mouledous, H Koivisto, J Ruuth, K Trontti, H Dhungana, M Keuters, I Weert, M Koskuvi, I Hovatta, AM Linden, C Rampon, T Malm, H Tanila, J Koistinaho, T Rolova
Cells, 2022-12-18;11(24):.
Species: Human
Sample Types: Tissue Homogenates
An in vitro workflow of neuron-laden agarose-laminin hydrogel for studying small molecule-induced amyloidogenic condition
Authors: P Namchaiw, P Bunreangsr, P Eiamcharoe, S Eiamboonse, R P Poo-Arpo
PLoS ONE, 2022-08-26;17(8):e0273458.
Species: Human
Sample Types: Cell Lysates
Blood circulating miR-28-5p and let-7d-5p associate with premature ageing in Down syndrome
Authors: C Morsiani, MG Bacalini, S Collura, M Moreno-Vil, N Breusing, A Bürkle, T Grune, C Franceschi, M De Eguileo, M Capri
Mechanisms of Ageing and Development, 2022-06-30;206(0):111691.
Species: Human
Sample Types: Plasma
Recurrent moderate hypoglycemia accelerates the progression of cognitive deficits through impairment of TRPC6/GLUT3 pathway in diabetic APP/PS1 mice
Authors: C He, Q Li, Y Cui, P Gao, W Shu, Q Zhou, L Wang, L Li, Z Lu, Y Zhao, H Ma, X Chen, H Jia, H Zheng, G Yang, D Liu, M Tepel, Z Zhu
JCI Insight, 2022-03-08;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates
Abeta peptides disrupt the barrier integrity and glucose metabolism of human induced pluripotent stem cell-derived brain microvascular endothelial cells
Authors: S Raut, R Patel, I Pervaiz, AJ Al-Ahmad
Neurotoxicology, 2022-01-19;89(0):110-120.
Species: Human
Sample Types: Cell Culture Supernates
Microglial Calhm2 regulates neuroinflammation and contributes to Alzheimer's disease pathology
Authors: J Cheng, Y Dong, J Ma, R Pan, Y Liao, X Kong, X Li, S Li, P Chen, L Wang, Y Yu, Z Yuan
Science Advances, 2021-08-25;7(35):.
Species: Mouse
Sample Types: Tissue Homogenates

Regulation of beta-amyloid production in neurons by astrocyte-derived cholesterol
Authors: H Wang, JA Kulas, C Wang, DM Holtzman, HA Ferris, SB Hansen
Proceedings of the National Academy of Sciences of the United States of America, 2021-08-17;118(33):.
Species: Mouse
Sample Types: Tissue Homogenates

APOE2 mitigates disease-related phenotypes in an isogenic hiPSC-based model of Alzheimer's disease
Authors: N Brookhouse, S Raman, C Frisch, G Srinivasan, DA Brafman
Molecular Psychiatry, 2021-04-09;0(0):.
Species: Human
Sample Types: Cell Culture Supernates

The pleiotropic beneficial intervention of olive oil intake on the Alzheimer's disease onset via fibrinolytic system
Authors: EE Tzekaki, M Tsolaki, &alphaA Pantazaki, G Geromichal, E Lazarou, M Kozori, Z Sinakos
Experimental gerontology, 2021-04-06;150(0):111344.
Species: Human
Sample Types: Serum

Extracellular vesicle-mediated amyloid transfer to neural progenitor cells: implications for RAGE and HIV infection
Authors: IE András, M Garcia-Con, C Yanick, P Perez, B Sewell, L Durand, M Toborek
Mol Brain, 2020-02-17;13(1):21.
Species: Human
Sample Types: Vesicles

Urolithin A attenuates memory impairment and neuroinflammation in APP/PS1 mice
Authors: Z Gong, J Huang, B Xu, Z Ou, L Zhang, X Lin, X Ye, X Kong, D Long, X Sun, X He, L Xu, Q Li, A Xuan
J Neuroinflammation, 2019-03-14;16(1):62.
Species: Mouse
Sample Types: Tissue Homogenates

The neuroprotective effect of deep brain stimulation at nucleus basalis of Meynert in transgenic mice with Alzheimer's disease
Authors: C Huang, H Chu, Y Ma, Z Zhou, C Dai, X Huang, L Fang, Q Ao, D Huang
Brain Stimul, 2018-08-27;0(0):.
Species: Mouse
Sample Types: Cell Lysates

The Isoquinoline Alkaloid Dauricine Targets Multiple Molecular Pathways to Ameliorate Alzheimer-Like Pathological Changes In Vitro
Authors: P Liu, X Chen, H Zhou, L Wang, Z Zhang, X Ren, F Zhu, Y Guo, X Huang, J Liu, PS Spencer, X Yang
Oxid Med Cell Longev, 2018-07-02;2018(0):2025914.
Species: Mouse
Sample Types: Cell Lysates

HDAC3 negatively regulates spatial memory in a mouse model of Alzheimer's disease
Authors: X Zhu, S Wang, L Yu, J Jin, X Ye, Y Liu, Y Xu
Aging Cell, 2017-08-03;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates

Folic acid supplementation improves cognitive function by reducing the levels of peripheral inflammatory cytokines in elderly Chinese subjects with MCI
Sci Rep, 2016-11-23;6(0):37486.
Species: Human
Sample Types: Serum