Human Androgen R/NR3C4 Antibody

Detection of Human Androgen R/NR3C4 by Western Blot. Western blot shows lysates of LNCaP human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Androgen R/NR3C4 Monoclonal Antibody (Catalog # MAB58762) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for Androgen R/NR3C4 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Androgen R/NR3C4 in Human LNCaP Cell Line. Androgen R/NR3C4 was detected in immersion fixed LNCaP human prostate cancer cell line (left panel; positive staining) and PC‑3 human prostate cancer cell line (right panel; negative staining) using Rabbit Anti-Human Androgen R/NR3C4 Monoclonal Antibody (Catalog # MAB58762) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; NL004) and counterstained with DAPI (blue). Specific staining was localized to the nucleus. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Androgen R/NR3C4 in Human Prostate Cancer. Androgen R/NR3C4 was detected in immersion fixed paraffin-embedded sections of human prostate cancer using Rabbit Anti-Human Androgen R/NR3C4 Monoclonal Antibody (Catalog # MAB58762) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of Androgen R/ NR3C4 in LNCaP Human Cell Line by Flow Cytometry. LNCaP human prostate cancer cell line was stained with Rabbit Anti-Human Androgen R/NR3C4 Monoclonal Antibody (Catalog # MAB58762, filled histogram) or isotype control antibody (MAB1050, open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (F0111). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). Staining was performed using our Staining Intracellular Molecules protocol.

Detection of Human Androgen R/NR3C4 by Simple Western^TM. Simple Western lane view shows lysates of LNCaP human prostate cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for Androgen R/NR3C4 at approximately 121 kDa (as indicated) using 10 µg/mL of Rabbit Anti-Human Androgen R/NR3C4 Monoclonal Antibody (Catalog # MAB58762). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.