Human Angiopoietin‑1 Biotinylated Antibody

Catalog #: BAF923
4 Citations Datasheet / COA / SDS

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BAF923

Product Details

Citations (4)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Angiopoietin‑1 Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Angiopoietin-1 in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross-reactivity with recombinant human (rh) Angiopoietin-2, rmAngiopoietin-3, and rhAngiopoietin-4 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Angiopoietin-1 (R&D Systems, Catalog # 923-AN)
Ser20-Phe498
Accession # Q5HYA0

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Angiopoietin‑1 (Catalog # 923-AN)

Human Angiopoietin-1 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.1-0.4 µg/mL

Use in combination with:

Capture Reagent: Human Angiopoietin‑1 Antibody (Catalog # MAB9231)

Standard: Recombinant Human Angiopoietin-1 Protein (Catalog # 923-AN)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Angiopoietin-1

Angiopoietin-1 (Ang-1) and Angiopoietin-2 (Ang-2) are two closely related secreted ligands which bind with similar affinity to Tie-2, a receptor tyrosine kinase with immunoglobulin and epidermal growth factor homology domains expressed primarily on endothelial cells and early hematopoietic cells. Tie-2 and angiopoietins have been shown to play critical roles in embryogenic angiogenesis and in maintaining the integrity of the adult vasculature (1).

Ang-1 cDNA encodes a 498 amino acid (aa) residue precursor protein that contains a coiled-coiled domain near the amino-terminus and a fibrinogen-like domain at the C-terminus. Human Ang-1 shares approximately 97% and 60% amino acid sequence identity with mouse Ang-1 and human Ang-2, respectively (1, 2). Ang-1 activates Tie-2 signaling on endothelial cells to promote chemotaxis, cell survival, cell sprouting, vessel growth and stabilization (1, 3, 4). Ang-2 has alternatively been reported to be an antagonist for Ang-1 induced Tie-2 signaling as well as an agonist for Tie-2 signaling, depending on the cell context (5).

References

Jones, N. et al. (2001) Nat. Rev. Mol. Cell Biol. 2:257.
Davis, S. et al. (1996) Cell 87:1161.
Witzenbichler, B. et al. (1998) J. Biol. Chem. 273:18514.
Papapetropoulos, A. et al. (1999) Lab. Inest. 79:213.
Teichert-Kuliszewska, K. et al. (2001) Cardiovasc. Res. 49:659.

Entrez Gene IDs

284 (Human); 11600 (Mouse)

Alternate Names

AGP1; AGPT; Ang1; ANG-1; angiopoietin 1; Angiopoietin-1; ANGPT1; KIAA0003

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human Angiopoietin‑1 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Angiopoietins bind thrombomodulin and inhibit its function as a thrombin cofactor
Authors: C Daly, X Qian, C Castanaro, E Pasnikowsk, X Jiang, BR Thomson, SE Quaggin, N Papadopoul, Y Wei, JS Rudge, G Thurston, GD Yancopoulo, S Davis
Sci Rep, 2018-01-11;8(1):505.
Species: Mouse
Sample Types: Plasma
Applications: ELISA Development (Detection)
Angiogenesis markers (VEGF, soluble receptor of VEGF and angiopoietin-1) in very early arthritis and their association with inflammation and joint destruction.
Authors: Clavel G, Bessis N, Lemeiter D, Fardellone P, Mejjad O, Menard JF, Pouplin S, Boumier P, Vittecoq O, Le Loet X, Boissier MC
Clin. Immunol., 2007-06-08;124(2):158-64.
Species: Human
Sample Types: Serum
Applications: ELISA Development
Angiopoietin-2 levels are elevated in exudative pleural effusions.
Authors: Kalomenidis I, Kollintza A, Sigala I, Papapetropoulos A, Papiris S, Light RW, Roussos C
Chest, 2006-05-01;129(5):1259-66.
Species: Human
Sample Types: Serum
Applications: ELISA Development
Plasma angiopoietin-1, angiopoietin-2 and Tie-2 in breast and prostate cancer: a comparison with VEGF and Flt-1.
Authors: Caine GJ, Blann AD, Stonelake PS, Ryan P, Lip GY
Eur. J. Clin. Invest., 2003-10-01;33(10):883-90.
Species: Human
Sample Types: Plasma
Applications: ELISA Development