Human AP-2 beta Antibody

Catalog #: AF5634 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5634
AF5634-SP

Detection of Human AP‑2 beta by Western Blot.

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Human AP-2 beta Antibody Summary

Species Reactivity

Human

Specificity

Detects endogenous human AP-2 beta in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human AP-2 beta
Leu121-Phe218
Accession # Q92481

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human AP‑2 beta by Western Blot. Western blot shows lysates of SH-SY5Y human neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Human AP-2 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5634) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for AP-2 beta at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: AP-2 beta

AP-2 beta (Activating enhancer-binding protein 2-beta; also TFAP2B) is a 52 kDa member of the AP-2 family of transcription factors. It is primarily a fetal-expressed protein, being found in renal and skin epithelial cells, as well as neuroblasts plus cells of the facial mesenchyme. In adults, AP-2 beta is expressed in renal epithelium. Human AP-2 beta is 460 amino acids (aa) in length. It contains a transactivation domain (aa 41‑131), a DNA-binding motif (aa 132‑298) and a C-terminal dimerization region (aa 299‑439). SUMOylation occurs on Lys21, adding 15 kDa of MW in SDS-PAGE. AP-2 beta both homodimerizes, and heterodimerizes with AP-2 alpha and -2 gamma. Dimers appear important for facial and limb development.

Long Name

Activating Enhancer-binding Protein 2 beta

Entrez Gene IDs

7021 (Human); 21419 (Mouse); 301285 (Rat)

Alternate Names

activating enhancer binding protein 2 beta; Activating enhancer-binding protein 2-beta; AP2 beta; AP-2 beta; AP-2B; AP2-B; AP2-beta; CHAR; MGC21381; TFAP2B; transcription factor AP-2 beta (activating enhancer binding protein 2 beta); transcription factor AP-2 beta (activating enhancer-binding protein 2 beta); transcription factor AP-2-beta