Human APBA3 Antibody

Catalog #: AF5689 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5689
AF5689-SP

Detection of Human APBA3 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human APBA3 Antibody Summary

Species Reactivity

Human

Specificity

Detects human APBA3 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant human (rh) X11‑ alpha and rhX11-beta is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human APBA3
Met1-Leu138
Accession # O96018

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human APBA3 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human APBA3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5689) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for APBA3 at approximately 96 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: APBA3

X11-gamma (Adaptor protein X11 gamma; also APBA3, Mint3 and X11L2) is a member of the X11 family of adaptor proteins. Unlike X11 alpha and -beta which are generally neuronal proteins, X11-gamma is widely expressed in all tissues. X11-gamma has many functions, including MT-MMP and Furin intracellular trafficking and APP binding. Human X11-gamma is 575 amino acids (aa) in length. It contains a poly-Ser segment (aa 165‑171), a PID region (aa 217‑381) that binds beta -amyloid precursor, and two PDZ domains (aa 393‑478 and 485‑560) that bind Arf GTPases. There is one potential splice variant that shows a 34 aa insertion after Ile505. Over aa 1‑138, human X11‑ gamma shares 62% aa identity with mouse X11‑ gamma.

Long Name

Amyloid Beta [A4] Precursor Protein-binding, Family A, Member 3

Entrez Gene IDs

9546 (Human); 57267 (Mouse); 83611 (Rat)

Alternate Names

Adapter protein X11gamma; amyloid beta (A4) precursor protein-binding, family A, member 3 (X11-like 2); amyloid beta (A4) precursor protein-binding, family A, member 3; APBA3; MGC:15815; MINT3; mint-3; Neuronal Munc18-1-interacting protein 3; Neuron-specific X11L2 protein; phosphotyrosine-binding/-interacting domain (PTB)-bearing protein; X11L2; X11L2amyloid beta A4 precursor protein-binding family A member 3; X11-like 2 protein