Human APLP-1 Antibody Summary
Gln34-Glu580
Accession # P51693
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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APLP‑1 in Human Brain. APLP‑1 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Goat Anti-Human APLP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3129) at 0.3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. Staining was performed using out protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
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Detection of Human and Mouse APLP‑1 by Simple WesternTM. Simple Western lane view shows lysates of human brain (hippocampus) and mouse brain (hippocampus), loaded at 0.2 mg/mL. A specific band was detected for APLP‑1 at approximately 119 kDa (as indicated) using 25 µg/mL of Goat Anti-Human APLP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3129). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: APLP-1
APLP-1 is a transmembrane metalloprotein that is expressed in central neurons. Similar to APP and APLP-2, APLP-1 is susceptible to cleavage by various secretases, generating multiple fragments from the extracellular and intracellular domains. These include peptides similar to the amyloidogenic A beta peptides and a cytoplasmic fragment that associates with Fe65 family proteins and functions as a transcriptional activator. The extracellular domain contains heparin and collagen binding regions and is 89% identical between human and mouse.
Product Datasheets
Citations for Human APLP-1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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APLP1 is endoproteolytically cleaved by ?-secretase without previous ectodomain shedding
Authors: L Schauenbur, F Liebsch, M Eravci, MC Mayer, C Weise, G Multhaup
Sci Rep, 2018-01-30;8(1):1916.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot -
Tau and other proteins found in Alzheimer's disease spinal fluid are linked to retromer-mediated endosomal traffic in mice and humans.
Authors: Simoes S, Neufeld J. L, et al.
Sci Transl Med
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