Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Apolipoprotein B/ApoB

ApoB is a component of LDL and VLDL particles and chylomicans. ApoB100, the full-length ApoB synthesized in the liver, is a 512 kDa secreted palmetoylated glycoprotein that contains 4536 amino acid residues. A truncated ApoB designated ApoB48, containing the N-terminal 48% of ApoB100, is also produced by the intestine as a result of post-transcriptional modification of the ApoB mRNA. Additionally, C-terminal truncated ApoB, varying in length from ApoB25 to ApoB89, exists as a result of mutations in the ApoB gene. Within the region used as the immunogen, the amino acid sequence of the human peptide is 80% and 82% identical to the mouse and canine sequence, respectively.

Entrez Gene IDs

338 (Human); 238055 (Mouse); 54225 (Rat)

Alternate Names

Apo B-100; APOB; ApoB-100; ApoB-48; apolipoprotein B (including Ag(x) antigen); Apolipoprotein B; apolipoprotein B-100; apolipoprotein B48; FLDB; LDLCQ4; mutant Apo B 100

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human Apolipoprotein B/ApoB Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Elevated apolipoprotein C3 augments diabetic kidney disease and associated atherosclerosis in type 2 diabetes
Authors: Cervantes, J;Koska, J;Kramer, F;Akilesh, S;Alpers, CE;Mullick, AE;Reaven, P;Kanter, JE;
JCI insight
Species: Human, Mouse
Sample Types: Whole Tissue
Applications: Immunohistochemistry
Increased apolipoprotein C3 drives cardiovascular risk in type 1 diabetes
Authors: JE Kanter, B Shao, F Kramer, S Barnhart, M Shimizu-Al, T Vaisar, MJ Graham, RM Crooke, CR Manuel, RA Haeusler, D Mar, K Bomsztyk, JE Hokanson, GL Kinney, JK Snell-Berg, JW Heinecke, KE Bornfeldt
J. Clin. Invest., 2019-07-11;130(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Intimal hyperplasia induced by vascular intervention causes lipoprotein retention and accelerated atherosclerosis
Authors: S Kijani, AM Vázquez, M Levin, J Borén, P Fogelstran
Physiol Rep, 2017-07-01;5(14):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Apolipoprotein C3 and apolipoprotein B colocalize in proximity to macrophages in atherosclerotic lesions in diabetes
Authors: Jenny E. Kanter, Karin E. Bornfeldt
Journal of Lipid Research