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Human Apolipoprotein B100/ApoB100 Antibody

Catalog #: AF3260
8 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3260
AF3260-SP
Detection of Human Apolipoprotein B100/ApoB100 by Western Blot.
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Citations (8)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Apolipoprotein B100/ApoB100 Antibody Summary

Species Reactivity
Human
Specificity
Detects human ApoB100 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human ApoB100
Ile3374-Val3600
Accession # AAP72970
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
5 µg/mL
Human plasma

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Apolipoprotein B100/ApoB100 antibody by Western Blot. View Larger

Detection of Human Apolipoprotein B100/ApoB100 by Western Blot. Western blot shows human plasma. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Apolipoprotein B100/ApoB100 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3260) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Apolipoprotein B100/ApoB100 at approximately 500 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western View Larger

Detection of Human Apolipoprotein B100/ApoB100 by Simple WesternTM. Simple Western shows lysates of human plasma, loaded at 1:50. A specific band was detected for Apolipoprotein B100/ApoB100 at approximately 307 kDa (as indicated) using 5 µg/mL of Goat Anti-Human Apolipoprotein B100/ApoB100 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3260). This experiment was conducted under reducing conditions and using the 66-440kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Apolipoprotein B100/ApoB100

ApoB100 is a major serum Apolipoprotein synthesized by the liver. It is an important constituent of LDL, VLDL and IDL particles. It interacts with the LDL receptor and is involved in the delivery of cholesterol to tissues. Within the peptide sequence of the immunogen, human and mouse ApoB100 share 74% amino acid sequence identity. The sequence within the immunogen is absent in ApoB48, an alternative splice isoform that is exclusively synthesized by the gut. ApoB48 contains the amino terminal 47% of ApoB100 and lacks the LDL receptor region.

Entrez Gene IDs
338 (Human); 238055 (Mouse); 54225 (Rat)
Alternate Names
apoB-100;apoB-48;Apolipoprotein B-100;FCHL2;FLDB;LDLCQ4; Apolipoprotein B100

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Citations for Human Apolipoprotein B100/ApoB100 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Selective enrichment of plasma cell-free messenger RNA in cancer-associated extracellular vesicles
    Authors: Hyun Ji Kim, Matthew J. Rames, Florian Goncalves, C. Ward Kirschbaum, Breeshey Roskams-Hieter, Elias Spiliotopoulos et al.
    Communications Biology
  2. Comparison of the Hemocompatibility of an Axial and a Centrifugal Left Ventricular Assist Device in an In Vitro Test Circuit
    Authors: Patrick Borchers, Patrick Winnersbach, Sandra Kraemer, Christian Beckers, Eva Miriam Buhl, Steffen Leonhardt et al.
    Journal of Clinical Medicine
  3. Y‐RNA subtype ratios in plasma extracellular vesicles are cell type‐ specific and are candidate biomarkers for inflammatory diseases
    Authors: Tom A.P. Driedonks, Sanne Mol, Sanne de Bruin, Anna‐Linda Peters, Xiaogang Zhang, Marthe F.S. Lindenbergh et al.
    Journal of Extracellular Vesicles
  4. Functional and transcriptomic analysis of extracellular vesicles identifies calprotectin as a new prognostic marker in peripheral arterial disease (PAD)
    Authors: Goren Saenz‐Pipaon, Patxi San Martín, Núria Planell, Alberto Maillo, Susana Ravassa, Amaia Vilas‐Zornoza et al.
    Journal of Extracellular Vesicles
  5. A novel three step protocol to isolate extracellular vesicles from plasma or cell culture medium with both high yield and purity
    Authors: Xiaogang Zhang, Ellen G. F. Borg, A. Manuel Liaci, Harmjan R. Vos, Willem Stoorvogel
    Journal of Extracellular Vesicles
  6. Lipoprotein(a) Cellular Uptake Ex�Vivo and Hepatic Capture In�Vivo Is Insensitive to PCSK9 Inhibition With Alirocumab
    Authors: K Chemello, S Beeské, TT Trang Tran, V Blanchard, EF Villard, B Poirier, JC Le Bail, G Dargazanli, S Ho-Van-Gui, D Boulay, O Bergis, MP Pruniaux, M Croyal, P Janiak, E Guillot, G Lambert
    JACC Basic Transl Sci, 2020-05-06;5(6):549-557.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Intimal hyperplasia induced by vascular intervention causes lipoprotein retention and accelerated atherosclerosis
    Authors: S Kijani, AM Vázquez, M Levin, J Borén, P Fogelstran
    Physiol Rep, 2017-07-01;5(14):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Functional Assay
  8. MicroRNA-27a regulates lipid metabolism and inhibits hepatitis C virus replication in human hepatoma cells.
    Authors: Shirasaki T, Honda M, Shimakami T, Horii R, Yamashita T, Sakai Y, Sakai A, Okada H, Watanabe R, Murakami S, Yi M, Lemon S, Kaneko S
    J Virol, 2013-02-28;87(9):5270-86.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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