Human APP/Protease Nexin II Pan Specific Antibody

Catalog #: AF1168
3 Citations Datasheet / COA / SDS

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AF1168
AF1168-SP

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Citations (3)

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Human APP/Protease Nexin II Pan Specific Antibody Summary

Species Reactivity

Human

Specificity

Detects human APP695⁺¹ and human APP770 in direct ELISAs and Western blots. This antibody recognizes a common epitope present on APP695⁺¹ and APP770 found within the segments Leu18 - Arg288 or Pro365 - Arg411 of APP770 (Accession P05067).

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human APP 695⁺¹ frameshift mutant
Leu18-Arg336
Accession # NP_958817

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human APP⁺¹ Western Blot Standard (Catalog # WBC012)

Immunohistochemistry

1-15 µg/mL

Immersion fixed paraffin-embedded sections of human Alzheimer's disease brain

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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APP/Protease Nexin II in Human Brain. APP/Protease Nexin II was detected in immersion fixed paraffin-embedded sections of human brain (Alzheimer's disease cortex) using Goat Anti-Human APP/Protease Nexin II Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1168) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: APP

Amyloid precursor protein (APP) is a type I membrane protein with several human isoforms due to alternative splicing. APP-770, -751, and -733 contain a Kunitz protease inhibitor (KPI) domain (residue 291 - 342) and APP-695 does not. APP is a cell surface molecule with many functions. It can be processed proteolytically in two different pathways. In one pathway, beta - and gamma -secretase cleave at the beta site between residue 670 and 671 and the gamma site between residue 711 and 714 to produce beta ‑amyloid peptide (A beta 40 and A beta 42), a major component in plaques found in brains of patients with Alzheimer's disease (1). The other pathway involves alpha -secretase that cleaves residues between 687 and 688. It is antiamyloidogenic due to its benign character and the prevention of the A beta peptide formation (2). Soluble APP containing the KPI domain, also referred to as protease nexin II, is a potent inhibitor of serine proteases and may have additional functions. For example, it may regulate the contact face of blood coagulation and limit thrombosis specially in brain due to its localization and coagulation factor XI inhibiting activity (3, 4).

References

Haass, C. (2004) EMBO J. 23:483.
Lichtenthaler, S. F. and C. Haass (2004) J. Clin. Invest. 113:1384.
Badellino, K.O. and P.N. Walsh (2000) Biochemistry 39:4769.
Xu, F. et al. (2005) Proc. Natl. Acad. Sci USA. 102:18135.

Long Name

Amyloid Precursor Protein

Entrez Gene IDs

351 (Human); 11820 (Mouse)

Alternate Names

amyloid beta (A4) precursor protein-binding, family B, member 2; amyloid beta A4 precursor protein-binding family B member 2; Amyloid beta precursor protein; Amyloid beta; APP; beta Amyloid; Protease Nexin II

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Citations for Human APP/Protease Nexin II Pan Specific Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Absence of microglia promotes diverse pathologies and early lethality in Alzheimer's disease mice
Authors: S Kiani Shab, S Morabito, EP Danhash, A McQuade, JR Sanchez, E Miyoshi, JP Chadarevia, C Claes, MA Coburn, J Hasselmann, J Hidalgo, KN Tran, AC Martini, W Chang Roth, J Pascual, E Head, DA Hume, C Pridans, H Davtyan, V Swarup, M Blurton-Jo
Cell Reports, 2022-06-14;39(11):110961.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot
A small molecule ApoE4-targeted therapeutic candidate that normalizes sirtuin 1 levels and improves cognition in an Alzheimer's disease mouse model
Authors: J Campagna, P Spilman, B Jagodzinsk, D Bai, A Hatami, C Zhu, T Bilousova, M Jun, CJ Elias, J Pham, G Cole, MJ LaDu, ME Jung, DE Bredesen, V John
Sci Rep, 2018-12-04;8(1):17574.
Species: Mouse
Sample Types: Plasma
Applications: ELISA Development
Sortilin and SorLA Display Distinct Roles in Processing and Trafficking of Amyloid Precursor Protein
Authors: Camilla Gustafsen, Simon Glerup, Lone Tjener Pallesen, Ditte Olsen, Olav M. Andersen, Anders Nykjær et al.
The Journal of Neuroscience