Human APPL Antibody

Catalog #: MAB7075 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB7075
MAB7075-SP

Detection of Human and Mouse APPL by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human APPL Antibody Summary

Species Reactivity

Human

Specificity

Detects human APPL in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG_2B Clone # 709208

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human APPL
Met1-Ile373
Accession # Q9UKG1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human and Mouse APPL by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HEK293 human embryonic kidney cell line, beta TC-6 mouse beta cell insulinoma cell line, OVCAR-3 human ovarian carcinoma cell line, and SH-SY5Y human neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human APPL Monoclonal Antibody (Catalog # MAB7075) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for APPL at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: APPL

APPL, also known as DCC interacting protein 13 alpha (DIP13 alpha), is a widely expressed approximately 80 kDa intracellular adaptor protein. APPL contains two coiled-coil domains (aa 215-259 and aa 621-673), a pleckstrin homology domain (aa 277-375) and a phosphotyrosine interaction domain (PID) (aa 496-656). These domains and the cytoplasmic and nuclear localization of APPL enable it to associate with a range of molecules involved in multiple pathways. APPL mediates the insulin-sensitizing and anti-inflammatory effects of Adiponectin through direct interactions with AdipoR1 and AdipoR2. Within aa 2-373, human APPL shares 99% aa sequence identity with mouse and rat APPL.

Long Name

Adapter Protein Containing PH Domain PTB Domain and Leucine Zipper Motif 1

Entrez Gene IDs

26060 (Human); 72993 (Mouse)

Alternate Names

Adapter protein containing PH domain, PTB domain and leucine zipper motif 1; adaptor protein containing pH domain, PTB domain and leucine zipper motif 1; adaptor protein, phosphotyrosine interaction, PH domain and leucine zippercontaining 1; AKT2 interactor; APPL; APPL1; APPLdip13-alpha; DCC-interacting protein 13-alpha; DIP 13 alpha; DIP13A; DIP13alpha; Dip13-alpha; KIAA1428; signaling adaptor protein DIP13alpha