Human Arginase 2/ARG2 Antibody

Name: Human Arginase 2/ARG2 Antibody
Brand: R&D Systems
SKU: MAB10602
Price: 370 USD

Catalog #: MAB10602 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Discontinued Product

MAB10602 has been discontinued.

View all Arginase 2/ARG2 products.

Detection of Human Arginase 2/ARG2 by Western Blot.

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All Arginase 2/ARG2 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Arginase 2/ARG2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Arginase 2/ARG2 in direct ELISAs.

Source

Recombinant Monoclonal Rabbit IgG Clone # 2611B

Purification

Protein A or G purified from cell culture supernatant

Immunogen

E. coli-derived human Arginase 2/ARG2
Val23-Ile354
Accession # P78540

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.05 µg/mL

HepG2 human hepatocellular carcinoma cell line

Simple Western

5 µg/mL

LNCaP human prostate cancer cell line and HepG2 human hepatocellular carcinoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Arginase 2/ARG2 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 0.05 µg/mL of Rabbit Anti-Human Arginase 2/ARG2 Monoclonal Antibody (Catalog # MAB10602) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for Arginase 2/ARG2 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Simple Western

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Detection of Human Arginase 2/ARG2 by Simple Western^TM. Simple Western lane view shows lysates of LNCaP human prostate cancer cell line and HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Arginase 2/ARG2 at approximately 40 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human Arginase 2/ARG2 Monoclonal Antibody (Catalog # MAB10602). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Arginase 2/ARG2

Arginase-2, a binuclear manganese-dependent metalloenzyme, is an approximately 36 kDa protein that forms a trimeric active molecule that catalyses the conversion of L-arginine into L-ornithine and urea (1). There are two isoforms of human arginase that share 58% sequence identity. Arginases are critical to the urea cycle and also influence downstream nitric oxide and polyamine levels (2) and hence are implicated to play a role in a number of diseases. While Arginase 1 is cytosolic and expressed predominantly in the liver, Arginase 2 is a mitochondrial enzyme expressed in several non-hepatic tissues including kidney, prostate and immune cells where it is regulated by inflammatory cytokines (2,3). Arginase 2 is implicated in regulation of a nitric oxide-dependent inflammatory response in lung and bronchial epithelial disease (4,5). It has been implicated in neuronal disease such as Huntington's disease due to nitric oxide signaling and suppressed enzymatic activity (6) and to play a role in atherosclerosis and hypertension through signaling pathways independent of its enzymatic activity (7,8). Arginase 2 promotes tumorigenesis through immune suppression, metabolism, cell proliferation and vascularization (9-11). Arginase-2 was noted as an attractive tumor therapeutic target (10) given that genetic deletion in mice does not lead to significant abnormalities (12).

References

Dowling, D. et al. (2008) Cell Mol. Life Sci. 65:2039.
Munder, M. (2009) Br. J. Pharmacol. 158:638.
Geiger, R. et al. (2016) Cell 167:829.
Kim, S.Y. et al. (2016) Sci. Rep. 6:29673.
Xu, W. et al. (2016) J. Clin. Invest. 126:2465.
Bichell, T.J.V. et al. (2017) Biochim. Biophys. Acta 1863:1596.
Jin, Y. et al. (2014) Am. J. Physiol. Lung Cell Mol. Physiol. 307:L197.
Xiong, Y. et al. (2014) Autophagy 10:2223.
Ino, Y. et al. (2013) PLoS One 8:e55146.
Zaytouni, T. et al. (2017) Nat. Commun. 8:242.
Costa, H. et al. (2016) Oncotarget 7:47221.
Shi, O. et al. (2001) Mol. Cell Biol. 21:811.

Long Name

Kidney-Type Arginase

Entrez Gene IDs

384 (Human); 11847 (Mouse); 29215 (Rat)

Alternate Names

ARG2; Arginase 2; arginase, type II; Arginase-2; arginase-2, mitochondrial; EC 3.5.3; EC 3.5.3.1; kidney arginase; Kidney-type arginase; L-arginine amidinohydrolase; L-arginine ureahydrolase; nonhepatic arginase; Non-hepatic arginase; Type II Arginase