Human ASCL2/Mash2 Antibody Summary
Met1-Ala49
Accession # Q99929
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human ASCL2/Mash2 by Western Blot. Western blot shows lysates of JAR human choriocarcinoma cell line and MCF-7 human breast cancer cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human ASCL2/Mash2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6539) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for ASCL2/Mash2 at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of ASCL2/Mash2 in Human Placenta. ASCL2/Mash2 was detected in immersion fixed paraffin-embedded sections of Human Placenta using Sheep Anti-Human ASCL2/Mash2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6539) at 10 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Sheep IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC006). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in extravillous trophoblast. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ASCL2/Mash2
ASCL2 (Achaete-scute like 2; also HASH2, bHLHa45 and Mash2) is a class II member of the HLH family of transcription factors. Its predicted MW is 20 kDa. ASCL2 shows restricted expression, being limited to intestinal Lgr5+ stem cells and first trimester placental cytotrophoblasts. In the intestine, ASCL2 is under the control of Wnts and serves to maintain an epithelium stem cell pool. In the placenta, ASCL2 acts to maintain the pool of cytotrophoblasts at the expense of synctiotrophoblasts, thus promoting placental growth. Human ASCL2 is 193 amino acids (aa) in length. It contains one poly-Arg motif (aa 36-39), a DNA binding sequence (aa 53-63) and an HLH domain (aa 64-103). ASCL2 forms heterodimers with daughterless homologs (E22, E2A and HEB). Over aa 1-49, human ASCL2 shares 59% aa identity with mouse Mash2.
Product Datasheets
Citations for Human ASCL2/Mash2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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Achaete-scute complex-like 2 regulated inflammatory mechanism through Toll-like receptor 4 activating in stomach adenocarcinoma
Authors: Enqi Zheng, Zhun Cai, Wangyong Li, Chuandou Ni, Qian Fang
World Journal of Surgical Oncology
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Intestinal Stem Cell Marker ASCL2 is a Novel Prognostic Predictor in Esophageal Adenocarcinoma
Authors: Yukiko Shibahara, Osvaldo Espin-Garcia, James Conner, Jessica Weiss, Mathieu Derouet, Jonathan Allen et al.
Cureus
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OVO-like 1 regulates progenitor cell fate in human trophoblast development.
Authors: Renaud S, Chakraborty D, Mason C, Rumi M, Vivian J, Soares M
Proc Natl Acad Sci U S A, 2015-10-26;112(45):E6175-84.
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did not work with citrate retrieval - not sure if the signal is real
