Human ATG10 Antibody

Catalog #: AF5464 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5464
AF5464-SP

Detection of Human ATG10 by Western Blot.

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Human ATG10 Antibody Summary

Species Reactivity

Human

Specificity

Detects endogenous human ATG10 in Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human ATG10
Met1-Thr190
Accession # Q9H0Y0

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human ATG10 by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line, HeLa human cervical epithelial carcinoma cell line, and HEK293 human embryonic kidney cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human ATG10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5464) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ATG10 at approximately 28 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ATG10

ATG10 (autophagy-related protein 10; also APG10-like) is a ubiquitous 28 kDa member of the ATG10 family of proteins. It serves as an E2-like enzyme during the initial stages of autophagosome formation by catalyzing the transfer of ATG12 to ATG5. This ATG5:ATG12 heterodimer subsequently associates non‑covalently with an ATG16 multimer to generate an autophagosome. Human ATG10 is 220 amino acids in length and contains an active site at Cys166 that forms a thiol ester bond with the C-terminal Gly of ATG12. There are multiple isoform variants. One shows a deletion of aa 37‑72, a second contains an alternate start site at Met43 that is accompanied by a 39 aa substitution for aa 152‑220, and a third shows a 53 aa substitution for aa 73‑220. Over amino acids 1‑190, human ATG10 shares 79% aa identity with mouse ATG10.

Long Name

Autophagy Related 10 Homolog

Entrez Gene IDs

83734 (Human); 66795 (Mouse); 688555 (Rat)

Alternate Names

APG10 autophagy 10-like (S. cerevisiae); APG10; APG10L; APG10-like; ATG10 autophagy related 10 homolog (S. cerevisiae); ATG10; Autophagy-related protein 10; DKFZp586I0418; EC 6.3.2.-; FLJ13954; PP12616; ubiquitin-like-conjugating enzyme ATG10