Human B7-H3 Fluorescein-conjugated Antibody

Catalog #: FAB1027F
3 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB1027F

Detection of B7‑H3 in PC‑3 Human Cell Line by Flow Cytometry.

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All B7-H3 Antibodies

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Citations (3)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human B7-H3 Fluorescein-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human B7-H3 in direct ELISAs and Western blots. In Western blots, does not cross-react with recombinant human (rh) B7‑H1, rhB7‑H2, rhB7‑1, rhB7‑2, or recombinant mouse PD-L2.

Source

Monoclonal Mouse IgG₁ Clone # 185504

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human B7‑H3
Leu29-Pro245
Accession # NP_079516

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of B7‑H3 in PC‑3 Human Cell Line by Flow Cytometry. PC-3 human prostate cancer cell line was stained with Mouse Anti-Human B7-H3 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB1027F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: B7-H3

B7-H3 is a member of the B7 family of immune costimulatory receptors. Its expression is induced on dendritic cells and monocytes by inflammatory cytokines.

Long Name

B7 Homolog 3

Entrez Gene IDs

80381 (Human); 102657 (Mouse); 315716 (Rat); 102131295 (Cynomolgus Monkey)

Alternate Names

B7H3; B7-H3; B7H34Ig-B7-H3; B7-H3B7 homolog 3; CD276 antigen; CD276 molecule; CD276; Costimulatory molecule

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human B7-H3 Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Fibronectin enhances tumor metastasis through B7-H3 in clear cell renal cell carcinoma
Authors: J Xie, M Sun, D Zhang, C Chen, S Lin, G Zhang
FEBS Open Bio, 2021-10-03;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Co-Immunoprecipitation, Western Blot
Idelalisib and caffeine reduce suppression of T cell responses mediated by activated chronic lymphocytic leukemia cells
Authors: BD Hock, SA MacPherson, JL McKenzie
PLoS ONE, 2017-03-03;12(3):e0172858.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
The cell surface proteome of human mesenchymal stromal cells.
Authors: Niehage C, Steenblock C, Pursche T, Bornhauser M, Corbeil D, Hoflack B
PLoS ONE, 2011-05-26;6(5):e20399.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry