Human B7-H6 Antibody

Catalog #: MAB71445 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB71445-100
MAB71445-SP

NKp30 Binding to B7-H6 Blocked by Human B7-H6 Antibody

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Human B7-H6 Antibody Summary

Species Reactivity

Human

Specificity

Detects human B7-H6 in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 1018415

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human embryonic kidney cell HEK293-derived human B7-H6
Asp25-Ser262
Accession # Q68D85

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Blockade of Receptor-ligand Interaction

In a functional ELISA, 20-120 ng/mL of Mouse Anti-Human B7-H6 Monoclonal Antibody (Catalog # MAB71445) will block 30% of the binding of 20 ng/mL of Recombinant Human NKp30 Fc Chimera Protein (Catalog # 1849-NK) to immobilized Recombinant Human B7-H6 His-tag Protein (Catalog # 9309-B7) coated at 1 μg/mL (100 µL/well). At 1 μg/mL, this antibody will block >50% of the binding.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Blockade of Receptor-ligand Interaction

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NKp30 Binding to B7-H6 Blocked by Human B7-H6 Antibody In a functional ELISA, 20-120 ng/mL of Mouse Anti-Human B7-H6 Monoclonal Antibody (Catalog # MAB71445) will block 30% of the binding of 20 ng/mL of Recombinant Human NKp30 Fc Chimera Protein (1849-NK) to immobilized Recombinant Human B7-H6 His-tag Protein (9309-B7) coated at 1 μg/mL (100 µL/well). At 1 μg/mL, this antibody will block >50% of the binding.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: B7-H6

B7-H6 is a glycosylated member of the B7 family of immune co-stimulatory proteins (1 2). Mature human B7-H6 consists of a 238 amino acid (aa) extracellular domain (ECD) that contains one Ig-like V domain and one Ig-like C1 domain, a 21 aa transmembrane segment, and a 171 aa cytoplasmic domain that contains one ITIM, one SH2, and one SH3 motif (3). Both of the Ig-like domains carry N-linked glycosylation (4). Within the ECD, human B7-H6 shares 99%, 94%, and 87% aa sequence identity with chimpanzee, orangutan, and gibbon B7-H6, respectively, and 53%-56% with bovine, canine, and equine B7-H6. Orthologs in mouse and rat have not been identified. The Ig-like V domain mediates 1:1 stoichiometric binding of B7-H6 to NKp30 expressed on NK cells (4, 5). It does not show binding to NKp44, NKp46, or NKG2D (3, 6). Ligation of NKp30 by B7-H6 induces NK cell activation and target cell cytolysis (3). B7-H6 is expressed on a wide range of hematopoietic, carcinoma, and melanoma tumor cells, which is consistent with the detection of NKp30 binding sites on many tumors (3, 7). The expression of NKp30 ligands on tumor cells correlates with tumor cell sensitivity to NKp30‑dependent cell lysis (7).

References