Human B7-H6 APC-conjugated Antibody Summary
Met1-Ser262
Accession # Q68D85
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of B7‑H6 in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human B7-H6 APC-conjugated Monoclonal Antibody (Catalog # FAB7144A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: B7-H6
B7-H6 is a glycosylated member of the B7 family of immune co‑stimulatory proteins (1, 2). Mature human B7-H6 consists of a 238 amino acid (aa) extracellular domain (ECD) that contains one Ig-like V domain and one Ig-like C1 domain, a 21 aa transmembrane segment, and a 171 aa cytoplasmic domain that contains one ITIM, one SH2, and one SH3 motif (3). Both of the Ig-like domains carry N-linked glycosylation (4). Within the ECD, human B7-H6 shares 99%, 94%, and 87% aa sequence identity with chimpanzee, orangutan, and gibbon B7-H6, respectively, and 53% - 56% with bovine, canine, and equine B7-H6. Orthologs in mouse and rat have not been identified. The Ig-like V domain mediates 1:1 stoichiometric binding of B7-H6 to NKp30 expressed on NK cells (4, 5). It does not show binding to NKp44, NKp46, or NKG2D (3, 6). Ligation of NKp30 by B7-H6 induces NK cell activation and target cell cytolysis (3). B7-H6 is expressed on a wide range of hematopoietic, carcinoma, and melanoma tumor cells, which is consistent with the detection of NKp30 binding sites on many tumors (3, 7). The expression of NKp30 ligands on tumor cells correlates with tumor cell sensitivity to NKp30‑dependent cell lysis (7).
- Zou, W. and L. Chen (2008) Nat. Rev. Immunol. 8:467.
- Bour-Jordan, H. et al. (2011) Immunol. Rev. 241:180.
- Brandt, C.S. et al. (2009) J. Exp. Med. 206:1495.
- Li, Y. et al. (2011) J. Exp. Med. 208:703.
- Joyce, M.G. et al. (2011) Proc. Natl. Acad. Sci. 108:6223.
- Arnon, T.I. et al. (2006) Semin. Cancer Biol. 16:348.
- Byrd, A. et al. (2007) PLoS ONE 2:e1339.
Product Datasheets
Citations for Human B7-H6 APC-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 7
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Comparison of characteristics and tumor targeting properties of extracellular vesicles derived from primary NK cells or NK-cell lines stimulated with IL-15 or IL-12/15/18
Authors: Miriam Aarsund, Filip M. Segers, Yunjie Wu, Marit Inngjerdingen
Cancer Immunology, Immunotherapy
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Differential IL-12 signaling induces human natural killer cell activating receptor-mediated ligand-specific expansion
Authors: Avishai Shemesh, Harry Pickering, Kole T. Roybal, Lewis L. Lanier
Journal of Experimental Medicine
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NKp30 and NKG2D contribute to natural killer recognition of HIV-infected cells
Authors: Zhao, NQ;Pi, R;Nguyen, DN;Ranganath, T;Seiler, C;Holmes, S;Marson, A;Blish, CA;
bioRxiv : the preprint server for biology
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
XPO1 inhibition sensitises CLL cells to NK cell mediated cytotoxicity and overcomes HLA-E expression
Authors: Fisher, JG;Doyle, ADP;Graham, LV;Sonar, S;Sale, B;Henderson, I;Del Rio, L;Johnson, PWM;Landesman, Y;Cragg, MS;Forconi, F;Walker, CJ;Khakoo, SI;Blunt, MD;
Leukemia
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Combination Blockade of the IL6R/STAT-3 Axis with TIGIT and Its Impact on the Functional Activity of NK Cells against Prostate Cancer Cells
Authors: S González-O, MC Tellez-Bañ, AS Méndez-Cle, A Bravo-Cuel, G Hernández, LA Palafox-Ma, J Haramati, EJ Pedraza-Br, K Sánchez-Re, PC Ortiz-Laza
Journal of Immunology Research, 2022-04-12;2022(0):1810804.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Genetic and Molecular Basis of Heterogeneous NK Cell Responses against Acute Leukemia
Authors: DR Makanga, F Da Rin de, G David, C Willem, L Dubreuil, N Legrand, T Guillaume, P Peterlin, A Lebourgeoi, MC Béné, A Garnier, P Chevallier, K Gendzekhad, A Cesbron, K Gagne, B Clemenceau, C Retière
Cancers (Basel), 2020-07-16;12(7):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Enhanced activation of human NK cells by drug-exposed hepatocytes
Authors: Frank Fasbender, Martin Obholzer, Sarah Metzler, Regina Stöber, Jan G. Hengstler, Carsten Watzl
Archives of Toxicology
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