Human B7-H6 PE-conjugated Antibody Summary
Met1-Ser262
Accession # Q68D85
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of B7‑H6 in K562 cells by Flow Cytometry. K562 cells were stained with Mouse Anti-Human B7‑H6 PE‑conjugated Monoclonal Antibody (Catalog # FAB7144P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: B7-H6
B7-H6 is a glycosylated member of the B7 family of immune co‑stimulatory proteins (1, 2). Mature human B7-H6 consists of a 238 amino acid (aa) extracellular domain (ECD) that contains one Ig-like V domain and one Ig-like C1 domain, a 21 aa transmembrane segment, and a 171 aa cytoplasmic domain that contains one ITIM, one SH2, and one SH3 motif (3). Both of the Ig-like domains carry N-linked glycosylation (4). Within the ECD, human B7-H6 shares 99%, 94%, and 87% aa sequence identity with chimpanzee, orangutan, and gibbon B7-H6, respectively, and 53% - 56% with bovine, canine, and equine B7-H6. Orthologs in mouse and rat have not been identified. The Ig-like V domain mediates 1:1 stoichiometric binding of B7-H6 to NKp30 expressed on NK cells (4, 5). It does not show binding to NKp44, NKp46, or NKG2D (3, 6). Ligation of NKp30 by B7-H6 induces NK cell activation and target cell cytolysis (3). B7-H6 is expressed on a wide range of hematopoietic, carcinoma, and melanoma tumor cells, which is consistent with the detection of NKp30 binding sites on many tumors (3, 7). The expression of NKp30 ligands on tumor cells correlates with tumor cell sensitivity to NKp30‑dependent cell lysis (7).
- Zou, W. and L. Chen (2008) Nat. Rev. Immunol. 8:467.
- Bour-Jordan, H. et al. (2011) Immunol. Rev. 241:180.
- Brandt, C.S. et al. (2009) J. Exp. Med. 206:1495.
- Li, Y. et al. (2011) J. Exp. Med. 208:703.
- Joyce, M.G. et al. (2011) Proc. Natl. Acad. Sci. 108:6223.
- Arnon, T.I. et al. (2006) Semin. Cancer Biol. 16:348.
- Byrd, A. et al. (2007) PLoS ONE 2:e1339.
Product Datasheets
Citations for Human B7-H6 PE-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 6
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CAR T-cell-mediated delivery of bispecific innate immune cell engagers for neuroblastoma
Authors: Pascual-Pasto, G;McIntyre, B;Hines, MG;Giudice, AM;Garcia-Gerique, L;Hoffmann, J;Mishra, P;Matlaga, S;Lombardi, S;Shraim, R;Schürch, PM;Yarmarkovich, M;Hofmann, TJ;Alikarami, F;Martinez, D;Tsang, M;Gil-de-Gómez, L;Spear, TT;Bernt, KM;Wolpaw, AJ;Dimitrov, DS;Li, W;Bosse, KR;
Nature communications
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Engineering of immune checkpoints B7-H3 and CD155 enhances immune compatibility of MHC-I-/- iPSCs for beta cell replacement
Authors: R Chimienti, T Baccega, S Torchio, F Manenti, S Pellegrini, A Cospito, A Amabile, MT Lombardo, P Monti, V Sordi, A Lombardo, M Malnati, L Piemonti
Cell Reports, 2022-09-27;40(13):111423.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
PVR and ICAM-1 on Blast Crisis CML Stem and Progenitor Cells with TKI Resistance Confer Susceptibility to NK Cells
Authors: N Kim, MY Kim, YU Cho, W Chen, KH Lee, HS Kim
Cancers (Basel), 2020-07-16;12(7):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
iPSC-Derived Platelets Depleted of HLA Class I Are Inert to Anti-HLA Class I and Natural Killer Cell Immunity
Authors: D Suzuki, C Flahou, N Yoshikawa, I Stirblyte, Y Hayashi, A Sawaguchi, M Akasaka, S Nakamura, N Higashi, H Xu, T Matsumoto, K Fujio, MG Manz, A Hotta, H Takizawa, K Eto, N Sugimoto
Stem Cell Reports, 2019-12-26;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Calreticulin exposure on malignant blasts correlates with improved natural killer cell-mediated cytotoxicity in acute myeloid leukemia patients
Authors: I Truxova, L Kasikova, C Salek, M Hensler, D Lysak, P Holicek, P Bilkova, M Holubova, X Chen, R Mikyskova, M Reinis, M Kovar, B Tomalova, JP Kline, L Galluzzi, R Spisek, J Fucikova
Haematologica, 2019-10-03;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Human NK Cells Lyse Th2-Polarizing Dendritic Cells via NKp30 and DNAM-1
Authors: K Walwyn-Bro, K Guldevall, M Saeed, D Pende, B Önfelt, AS MacDonald, DM Davis
J. Immunol., 2018-08-17;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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