Human BACH1 Antibody Summary
Ser133-Glu513
Accession # O14867
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human BACH1 by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and A172 human glioblastoma cell line. PVDF membrane was probed with 0.2 µg/mL of Human/Mouse BACH1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5776) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for BACH1 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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BACH1 in Human Lung Cancer Tissue. BACH1 was detected in immersion fixed paraffin-embedded sections of human lung cancer tissue using Human/Mouse BACH1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5776) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BACH1
BACH1 (BTB and CNC homolog 1; also basic leucine zipper transcription factor 1) is a ubiquitously expressed member of the Bach family of transcription factors. Although its predicted MW is 82 kDa, it runs anomalously at 110 kDa in SDS-PAGE. BACH1 forms non‑covalent homodimers, and heterodimers with Maf oncoproteins and p53-related proteins. It apparently serves as an architectural component for gene regulatory protein complexes. Human BACH1 is 736 amino acids (aa) in length. It contains a protein-interaction BTB domain (aa 24‑127), a DNA-binding motif (aa 562‑577), and a Leu-zipper domain (aa 585‑607). This molecule should not be confused with Bach1/Fancj/Brip1 helicase. There are two BACH1 splice variants with a 24 and 31 aa substitution for aa 593‑736, respectively. Over aa 133‑513, human BACH1 shares 75% aa identity with mouse BACH1.
Product Datasheets
Citations for Human BACH1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 8
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Effective breast cancer combination therapy targeting BACH1 and mitochondrial metabolism
Authors: J Lee, AE Yesilkanal, JP Wynne, C Frankenber, J Liu, J Yan, M Elbaz, DC Rabe, FD Rustandy, P Tiwari, EA Grossman, PC Hart, C Kang, SM Sanderson, J Andrade, DK Nomura, MG Bonini, JW Locasale, MR Rosner
Nature, 2019-03-06;0(0):.
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BACH1 regulates the differentiation of vascular smooth muscle cells from human embryonic stem cells via CARM1-mediated methylation of H3R17
Authors: He, Y;Guo, J;Yu, Y;Jin, J;Jiang, Q;Li, Q;Ma, S;Pan, Q;Lin, J;Jiang, N;Ma, J;Li, Y;Hou, Y;Zhi, X;Jiang, L;Qu, L;Osto, E;Wang, X;Wei, X;Meng, D;
Cell reports
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
BACH1-induced ferroptosis drives lymphatic metastasis by repressing the biosynthesis of monounsaturated fatty acids
Authors: X Xie, L Tian, Y Zhao, F Liu, S Dai, X Gu, Y Ye, L Zhou, X Liu, Y Sun, X Zhao
Oncogene, 2023-01-20;14(1):48.
Species: Human
Sample Types: Whole Cells
Applications: ChIP -
A Heme-Binding Transcription Factor BACH1 Regulates Lactate Catabolism Suggesting a Combined Therapy for Triple-Negative Breast Cancer
Authors: J Padilla, BS Lee, K Zhai, B Rentz, T Bobo, NM Dowling, J Lee
Cells, 2022-03-31;11(7):.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation -
BACH1 promotes the progression of esophageal squamous cell carcinoma by inducing the epithelial-mesenchymal transition and angiogenesis
Authors: Y Zhao, J Gao, X Xie, P Nan, F Liu, Y Sun, X Zhao
Cancer Medicine, 2021-05-01;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: ChIP -
Mild Oxidative Stress Reduces NRF2 SUMOylation to Promote Kras/Lkb1/Keap1 Mutant Lung Adenocarcinoma Cell Migration and Invasion
Authors: J Xu, H Guo, Z Xing, W Zhang, J He, J Cheng, R Cai
Oxidative Medicine and Cellular Longevity, 2020-11-24;2020(0):6240125.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Bach1 regulates self-renewal and impedes mesendodermal differentiation of human embryonic stem cells
Authors: X Wei, J Guo, Q Li, Q Jia, Q Jing, Y Li, B Zhou, J Chen, S Gao, X Zhang, M Jia, C Niu, W Yang, X Zhi, X Wang, D Yu, L Bai, L Wang, J Na, Y Zou, J Zhang, S Zhang, D Meng
Sci Adv, 2019-03-13;5(3):eaau7887.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Induction of heme oxygenase I (HMOX1) by HPP-4382: a novel modulator of Bach1 activity.
Authors: Attucks, Otis C, Jasmer, Kimberly, Hannink, Mark, Kassis, Jareer, Zhong, Zhenping, Gupta, Suparna, Victory, Sam F, Guzel, Mustafa, Polisetti, Dharma R, Andrews, Robert, Mjalli, Adnan M, Kostura, Matthew
PLoS ONE, 2014-07-14;9(7):e101044.
Species: Human
Sample Types:
Applications: Immunoprecipitation
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