Human BATF Antibody Summary
Met1-Pro125
Accession # Q16520
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human BATF by Western Blot. Western blot shows lysates of KARPAS 299 human anaplastic large cell lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human BATF Monoclonal Antibody (Catalog # MAB8054) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for BATF at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of BATF in Raji Human Cell Line by Flow Cytometry. Raji human Burkitt's lymphoma cell line was stained with Mouse Anti-Human BATF Monoclonal Antibody (Catalog # MAB8054, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BATF
BATF is an approximately 20 kDa member of the AP-1 family of basic leucine zipper transcription factors. It associates with c-Jun proteins to form heterodimeric factors that inhibit transcription at AP-1 sites. The DNA binding of BATF is regulated by its phosphorylation at Ser43. BATF plays a key role in several aspects of immune system development. It suppresses the differentiation of NKT and iNKT cells, while it promotes the differentiation of Th17, Th2, follicular Th cells, CD8+ T cells, and CD8+ dendritic cells. It is required for class-switch recombination in B cells and T cells as well as for germinal center formation and B cell maturation. It is also required for the expression of T cell surface proteins that mediate homing of Th cells to the gut. BATF cooperates with IRF4 in binding to composite DNA elements that are responsive to both IRF4 and AP1. Human BATF1 shares 96% amino acid sequence identity with mouse and rat BATF1.
Product Datasheets
Citation for Human BATF Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Type I interferon-enhanced IL-10 expression in human CD4 T cells is regulated by STAT3, STAT2, and BATF transcription factors
Authors: U Govender, B Corre, Y Bourdache, S Pellegrini, F Michel
J. Leukoc. Biol, 2017-02-27;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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