Human BATF3 Antibody

Catalog #: MAB7437
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB7437
MAB7437-SP

Detection of BATF3 in Human (PBMC) Monocytes by Flow Cytometry.

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All BATF3 Antibodies

Product Details

Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human BATF3 Antibody Summary

Species Reactivity

Human

Specificity

Detects human BATF3 in ELISA.

Source

Monoclonal Mouse IgG_2B Clone # 841702

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human BATF3
Met1-Arg127
Accession # Q9NR55

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells

Human peripheral blood mononuclear cell (PBMC) monocytes fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005); Jurkat human acute T cell leukemia cell line fixed and permeabilized with FlowX FoxP3/Transcription Factor Fixation & Perm Buffer kit (FC012)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of BATF3 antibody in Human (PBMC) Monocytes antibody by Flow Cytometry.

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Detection of BATF3 in Human (PBMC) Monocytes by Flow Cytometry. Human peripheral blood mononuclear cell (PBMC) monocytes were stained with Mouse Anti-Human CD11c PE-conjugated Monoclonal Antibody (FAB1777P) and either (A) Mouse Anti-Human BATF3 Monoclonal Antibody (Catalog # MAB7437) or (B) Mouse IgG_2BFlow Cytometry Isotype Control (MAB0041) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). View our protocol for Staining Intracellular Molecules.

Flow (Intracellular)

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Detection of BATF3 in Jurkat cells by Flow Cytometry Jurkat cells were stained with Mouse Anti-Human BATF3 Monoclonal Antibody (Catalog # MAB7437, filled histogram) or isotype control antibody (Catalog # MAB004, open histogram) followed by Fluorescein-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0103B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BATF3

BATF3 (Basic leucine zipper transcriptional factor ATF-like 3; also p21SNFT) is a 20 kDa nuclear member of the bZIP family of proteins. It is expressed in Th1 cells and conventional dendritic cells (CD11c⁺), and serves to downregulate AP-1 mediated transcription. BATF3 accomplishes this by heterodimerizing with Jun and binding to AP-1 consensus binding sites, thus precluding a Jun/Fos interaction with gene activation. Human BAFT3 is 127 amino acids (aa) in length. It contains one DNA binding motif (aa 41-59) with an adjacent leucine-zipper (aa 63-84), but lacks a transactivation domain. Full-length human BATF3 (aa 1-127) shares 80% aa sequence identity with mouse BATF3.

Long Name

Basic Leucine Zipper Transcription Factor, ATF-like 3

Entrez Gene IDs

55509 (Human); 381319 (Mouse); 60462 (Rat)

Alternate Names

basic leucine zipper transcription factor, ATF-like 3; basic leucine zipper transcriptional factor ATF-like 3; BATF3; B-ATF-3; FLJ36352; FLJ37535,21 kDa small nuclear factor isolated from T-cells; JDP1; Jun dimerization protein 1; Jun dimerization protein p21SNFT; JUNDM1; SNFT; SNFT21-kD small nuclear factor isolated from T cells

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citation for Human BATF3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Genomic programming of IRF4-expressing human Langerhans cells
Authors: S Sirvent, AF Vallejo, J Davies, K Clayton, Z Wu, J Woo, J Riddell, VK Chaudhri, P Stumpf, LA Nazlamova, G Wheway, M Rose-Zeril, J West, M Pujato, X Chen, CH Woelk, B MacArthur, M Ardern-Jon, PS Friedmann, MT Weirauch, H Singh, ME Polak
Nat Commun, 2020-01-16;11(1):313.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry