Human Bax Minus C-Terminus Antibody

Catalog #: AB820 Datasheet / COA / SDS

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AB820

Detection of Human Bax Minus C-Terminus by Western Blot.

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Human Bax Minus C-Terminus Antibody Summary

Species Reactivity

Human

Specificity

Detects human Bax Minus C-Terminus. Does not detect mouse Bax.

Source

Polyclonal Goat IgG

Purification

Protein A or G purified

Immunogen

Human Bax synthetic peptide

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Applications

Recommended Concentration

Sample

Western Blot

4 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

Immersion fixed paraffin-embedded sections of human breast

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Bax Minus C-Terminus by Western Blot. Western blot shows lysates of THP-1 human acute monocytic leukemia cell line. PVDF membrane was probed with 4 µg/mL Goat Anti-Human Bax Minus C-Terminus Polyclonal Antibody (Catalog # AB820) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). For additional reference, recombinant human Bax (1 ng) was included. A specific band for Bax was detected at approximately 21 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Bax

Bax belongs to the Bcl-2 protein family. It forms homodimers and heterodimers with Bcl-2 and functions as an apoptotic activator. Bax interacts with the mitochondrial voltage dependent anion channel and promotes the release of cytochrome c.

Long Name

Bcl Associated X Protein

Entrez Gene IDs

581 (Human); 12028 (Mouse); 24887 (Rat)

Alternate Names

apoptosis regulator BAX; Bax; BCL2-associated X protein; Bcl2-L-4; BCL2L4bcl2-L-4; Bcl-2-like protein 4