Human Bcl-9 Antibody

Catalog #: AF3996
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3996
AF3996-SP

Detection of Human Bcl‑9 by Western Blot.

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All Bcl-9 Antibodies

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Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Bcl-9 Antibody Summary

Species Reactivity

Human

Specificity

Detects endogenous human Bcl-9 in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Bcl-9
Met1009-Gly1328
Accession # O00512

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Bcl‑9 by Western Blot. Western blot shows lysates of HCT-116 human colorectal carcinoma cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Human Bcl-9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3996) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Bcl-9 at approximately 150 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Bcl-9

Bcl-9 (B-cell lymphoma 9; also Protein legless homolog) is a transcriptional regulator that belongs to the Bcl-9 family of proteins. It is expressed in multiple tissues and serves to recruit Pygopus to the Wnt-pathway beta -catenin-TCF complex in the nucleus. Bcl-9 and Bcl-9-2 are considered evolutionary duplicates of Legless that perform the same task with different regulation. Human Bcl-9 is 1426 amino acids (aa) in length. It contains one phosphothreonine and three phosphoserine sites that have been identified so far, two poly-Pro regions (aa 514‑517 and 970‑973), and one poly-Ala segment (aa 900‑903). There is one potential alternate start site at Met27, and a variant isoform exists that shows a four aa substitution for aa 1391‑1426. Over aa 1009‑1328, human Bcl-9 is 96% aa identical to mouse Bcl-9.

Long Name

B Cell Lymphoma/Leukemia 9, Nuclear Co-factor of beta-Catenin Signaling

Entrez Gene IDs

607 (Human); 77578 (Mouse); 310704 (Rat)

Alternate Names

B-cell CLL/lymphoma 9 protein; B-cell CLL/lymphoma 9; B-cell lymphoma 9 protein; Bcl9; Bcl-9; BCL9L; LGS; MGC131591; Protein legless homolog

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citation for Human Bcl-9 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Wnt status-dependent oncogenic role of BCL9 and BCL9L in hepatocellular carcinoma
Authors: Nicole Huge, Maria Sandbothe, Anna K. Schröder, Amelie Stalke, Marlies Eilers, Vera Schäffer et al.
Hepatology International