Human BCMA/TNFRSF17 Quantikine ELISA Kit

Catalog #: DBCMA0 Datasheet / COA / SDS

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PDBCMA0
DBCMA0
SBCMA0

Human BCMA/TNFRSF17 ELISA Standard Curve

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All BCMA/TNFRSF17 ELISAs

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Summary Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human BCMA/TNFRSF17 Quantikine ELISA Kit Summary

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Saliva (50 uL), Urine (50 uL)

Sensitivity

10.8 pg/mL

Assay Range

31.3 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine)

Specificity

Natural and recombinant human BCMA.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Human B Cell Maturation Antigen (BCMA) Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human BCMA in cell culture supernates, serum, plasma, saliva, and urine. It contains recombinant human BCMA and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human BCMA showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human BCMA.

Recovery

The recovery of human BCMA spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	99	80-106
Saliva (n=4)	96	83-115
Urine (n=4)	102	94-112

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human BCMA were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.

Scientific Data

N/A

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Human BCMA/TNFRSF17 ELISA Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: BCMA/TNFRSF17

B cell maturation protein (BCMA) is a member of the TNF receptor superfamily (TNFRSF17). BCMA is expressed in immune organs and mature Bcell lines. Although some expression has been observed at the cell surface, BCMA appears to be localized to the Golgi compartment. BCMA binding to APRIL or BAFF stimulates IgM production in peripheral blood B cells and increases the survival of cultured B cells. Mature human and mouse BCMA share 86% amino acid sequence identity.

Long Name:

B Cell Maturation Factor

Entrez Gene IDs:

608 (Human); 21935 (Mouse); 287034 (Rat); 102145399 (Cynomolgus Monkey)

Alternate Names:

B cell maturation antigen; B-cell maturation protein; BCMA; BCMAtumor necrosis factor receptor superfamily member 17; BCMB-cell maturation factor; CD269 antigen; CD269; TNFRSF13A; TNFRSF17; tumor necrosis factor receptor superfamily, member 17