Human BDNF Antibody
Discontinued Product
MAB848 has been discontinued and is replaced by MAB848R.
Human BDNF Antibody Summary
Arg128-Arg247
Accession # P23560
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BDNF
Brain-derived neurotrophic factor (BDNF) is a member of the NGF family of neurotrophic factors (also named neurotrophins) that are required for the differentiation and survival of specific neuronal subpopulations in both the central as well as the peripheral nervous system. The neurotrophin family is comprised of at least four proteins including NGF, BDNF, NT-3, and NT-4/5. These secreted cytokines are synthesized as prepropeptides that are proteolytically processed to generate the mature proteins. All neurotrophins have six conserved cysteine residues that are involved in the formation of three disulfide bonds and all share approximately 55% sequence identity at the amino acid level. Similarly to NGF, bioactive BDNF is predicted to be a non-covalently linked homodimer. BDNF cDNA encodes a 247 amino acid residue precursor protein with a signal peptide and a proprotein that are cleaved to yield the 119 amino acid residue mature BDNF. The amino acid sequence of mature BDNF is identical in all mammals examined. High levels of expression of BDNF have been detected in the hippocampus, cerebellum, fetal eye and placenta. In addition, low levels of BDNF expression are also found in the pituitary gland, spinal cord, heart, lung and skeletal muscle. BDNF binds with high affinity and specifically activates the TrkB tyrosine kinase receptor.
- Eide, F.F. et al. (1993) Exp. Neurol. 121:200.
- Snider, W.D. (1994) Cell 77:627.
- Barbacid, M. (1994) J. Neurobiol. 25:1386.
Product Datasheets
Citations for Human BDNF Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Illness severity and biomarkers in depression: Using a unidimensional rating scale to examine BDNF
Authors: MA Caldieraro, EA Vares, LH Souza, L Spanemberg, TA Guerra, B Wollenhaup, P Ferrari, AA Nierenberg, MP Fleck
Compr Psychiatry, 2017-03-06;75(0):46-52.
Species: Human
Sample Types: Serum
Applications: ELISA Development (Capture) -
Heterogeneous intracellular trafficking dynamics of brain-derived neurotrophic factor complexes in the neuronal soma revealed by single quantum dot tracking.
Authors: Vermehren-Schmaedick, Anke, Krueger, Wesley, Jacob, Thomas, Ramunno-Johnson, Damien, Balkowiec, Agnieszk, Lidke, Keith A, Vu, Tania Q
PLoS ONE, 2014-04-14;9(4):e95113.
Species: Rat
Sample Types: Cell Culture Supernates
Applications: ELISA Development (Capture) -
BDNF plasma levels in acute stroke.
Authors: Di Lazzaro V, Profice P, Pilato F, Dileone M, Florio L, Tonali PA, Angelucci F
Neurosci. Lett., 2007-06-08;422(2):128-30.
Species: Human
Sample Types: Plasma
Applications: ELISA Development
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The MAB648 antibody was used as the capture antibody along with MAB848 as the detection antibody for a sandwich ELISA assay. The calibrator used was recombinant Human BDNF (248-BD). Healthy human serum and plasma samples were all quantifiable at a 1:2 dilution.
