Human beta-1,3-N-acetylglucosaminyl-transferase 2/B3GNT2 Antibody

Catalog #: MAB3960 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB3960
MAB3960-SP

All Beta-1,3-N-Acetylglucosaminyltransferase 2/B3GNT2 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human beta-1,3-N-acetylglucosaminyl-transferase 2/B3GNT2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human beta -1,3-N-acetylglucosaminyltransferase 2/B3GNT2 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human B4GALT1 is observed

Source

Monoclonal Mouse IgG_2B Clone # 485327

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human beta -1,3-N-acetylglucosaminyltransferase 2/B3GNT2
Lys29-Cys397
Accession # Q9NY97

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human beta -1,3-N-acetylglucosaminyltransferase 2/B3GNT2 (Catalog # 3960-GT)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Beta-1,3-N-Acetylglucosaminyltransferase 2/B3GNT2

beta 1,3-Linked GlcNAc residues are present in the backbone of various biologically important glycans which are involved in many essential biological functions such as keratan sulfate synthesis in corneal tissue (1). The addition of such residues are catalyzed by a family of beta 1,3‑N‑acetylglucosaminyltransferases, that includes at least eight members (1‑5). All of them are type II Golgi resident transmembrane proteins and have high homology to the beta 1,3-galactosyltransferase family. beta 1,3‑N‑acetylglucosaminyltransferases 2 or beta 3GNT2 prefers the substrate lacto-N-neotetraose and is involved in the biosynthesis of poly-N-acetyllactosamine chains (6).

References

Seko, A. and Yamashita, K. (2005) Glycobiology 15:943.
Kataoka, K. and Huh, N.-H. (2002) Biochem. Biophys. Res. Commun. 294:843.
Iwai,T. et al. (2002) J. Biol. Chem. 277:12802.
Togayachi, A. et al. (2001) J. Biol. Chem. 276:22032.
Sasaki, K. et al. (1997) Proc. Natl. Acad. Sci. USA 94:14294.
Shiraishi, N. et al. (2001) J. Biol. Chem. 276:3498.

Long Name

UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyl-transferase 1

Entrez Gene IDs

10678 (Human); 53625 (Mouse)

Alternate Names

B3Galt6; B3GALT7; b3Gal-T7; B3GNT; B3GNT1; B3GN-T1; B3GNT2; B3GN-T2; B3GNT-2; Beta-1,3-galactosyltransferase 7; Beta-1,3-GalTase 7; Beta-1,3-Gn-T1; Beta-1,3-Gn-T2; Beta-1,3-N-acetylglucosaminyltransferase 1; Beta-1,3-N-acetylglucosaminyltransferase 2; beta-1,3-N-acetylglucosaminyltransferase bGnT-1; beta-1,3-N-acetylglucosaminyltransferase bGnT-2; Beta3GalT7; Beta3Gal-T7; BETA3GNT; Beta3gnt1; Beta3Gn-T1; Beta3Gn-T2; Beta-3-Gx-T7; BGnT-1; BGNT2; BGnT-2; EC 2.4.1.-; UDP-Gal:beta-GlcNAc beta-1,3-galactosyltransferase 7; UDP-galactose:beta-N-acetylglucosamine beta-1,3-galactosyltransferase 7; UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 1; UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2